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Literature summary for 1.11.1.19 extracted from

  • Salvachua, D.; Prieto, A.; Martinez, A.T.; Martinez, M.J.
    Characterization of a novel dye-decolorizing peroxidase (DyP)-type enzyme from Irpex lacteus and its application in enzymatic hydrolysis of wheat straw (2013), Appl. Environ. Microbiol., 79, 4316-4324.
    View publication on PubMed
Search for more literature for 1.11.1.19

Inhibitors

Inhibitors Comment Organism Structure
H2O2 maximum relative activity at final H2O2 concentrations between 0.4 and 0.8 mM, activity then gradually drops off. A residual activity of 30% still remains at 12.5 mM H2O2, and the enzyme is completely inactivated in the presence of 50 mM H2O2 Irpex lacteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0112
-
 Reactive Black 5 pH 3.0, temperature not specified in the publication Irpex lacteus
0.0135
-
 Reactive Blue 19 pH 4.0, temperature not specified in the publication Irpex lacteus
0.028
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3,0, temperature not specified in the publication Irpex lacteus
0.0726
-
 2,6-dimethoxyphenol pH 3.0, temperature not specified in the publication Irpex lacteus
0.0795
-
 H2O2 pH 3.0, temperature not specified in the publication Irpex lacteus
3.61
-
 veratryl alcohol pH 2.0, temperature not specified in the publication Irpex lacteus

Organism

Organism UniProt Comment Textmining
Irpex lacteus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37.8
-
 substrate 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate, pH 5.0, temperature not specified in the publication Irpex lacteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 2,6-dimethoxyphenol + H2O2
-
 Irpex lacteus coerulignone + 2 H2O
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Irpex lacteus ? + H2O
-
 ?
Reactive Black 5 + H2O2 + H+
-
 Irpex lacteus oxidized Reactive Black 5 + H2O
-
 ?
Reactive Blue 19 + H2O2 + H+
-
 Irpex lacteus oxidized Reactive Blue 19 + H2O
-
 ?
veratryl alcohol + H2O2 + H+
-
 Irpex lacteus veratraldehyde + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7
-
 veratryl alcohol pH 2.0, temperature not specified in the publication Irpex lacteus
11.9
-
 Reactive Black 5 pH 3.0, temperature not specified in the publication Irpex lacteus
70.9
-
 2,6-dimethoxyphenol pH 3.0, temperature not specified in the publication Irpex lacteus
79.9
-
 Reactive Blue 19 pH 4.0, temperature not specified in the publication Irpex lacteus
224
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3,0, temperature not specified in the publication Irpex lacteus
419
-
 H2O2 pH 3.0, temperature not specified in the publication Irpex lacteus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.83
-
 veratryl alcohol pH 2.0, temperature not specified in the publication Irpex lacteus
970
-
 2,6-dimethoxyphenol pH 3.0, temperature not specified in the publication Irpex lacteus
1100
-
 Reactive Black 5 pH 3.0, temperature not specified in the publication Irpex lacteus
5300
-
 H2O2 pH 3.0, temperature not specified in the publication Irpex lacteus
5900
-
 Reactive Blue 19 pH 4.0, temperature not specified in the publication Irpex lacteus