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Literature summary for 1.11.1.19 extracted from
- Characterization of a novel dye-decolorizing peroxidase (DyP)-type enzyme from Irpex lacteus and its application in enzymatic hydrolysis of wheat straw (2013), Appl. Environ. Microbiol., 79, 4316-4324.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | maximum relative activity at final H2O2 concentrations between 0.4 and 0.8 mM, activity then gradually drops off. A residual activity of 30% still remains at 12.5 mM H2O2, and the enzyme is completely inactivated in the presence of 50 mM H2O2 | Irpex lacteus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0112 | - |
Reactive Black 5 | pH 3.0, temperature not specified in the publication | Irpex lacteus | |
| 0.0135 | - |
Reactive Blue 19 | pH 4.0, temperature not specified in the publication | Irpex lacteus | |
| 0.028 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3,0, temperature not specified in the publication | Irpex lacteus | |
| 0.0726 | - |
2,6-dimethoxyphenol | pH 3.0, temperature not specified in the publication | Irpex lacteus | |
| 0.0795 | - |
H2O2 | pH 3.0, temperature not specified in the publication | Irpex lacteus | |
| 3.61 | - |
veratryl alcohol | pH 2.0, temperature not specified in the publication | Irpex lacteus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Irpex lacteus | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37.8 | - |
substrate 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate, pH 5.0, temperature not specified in the publication | Irpex lacteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 2,6-dimethoxyphenol + 2 H2O2 | - |
Irpex lacteus | coerulignone + 2 H2O | - |
? | |
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Irpex lacteus | ? + H2O | - |
? | |
| Reactive Black 5 + H2O2 + H+ | - |
Irpex lacteus | oxidized Reactive Black 5 + H2O | - |
? | |
| Reactive Blue 19 + H2O2 + H+ | - |
Irpex lacteus | oxidized Reactive Blue 19 + H2O | - |
? | |
| veratryl alcohol + H2O2 + H+ | - |
Irpex lacteus | veratraldehyde + H2O | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.7 | - |
veratryl alcohol | pH 2.0, temperature not specified in the publication | Irpex lacteus | |
| 11.9 | - |
Reactive Black 5 | pH 3.0, temperature not specified in the publication | Irpex lacteus | |
| 70.9 | - |
2,6-dimethoxyphenol | pH 3.0, temperature not specified in the publication | Irpex lacteus | |
| 79.9 | - |
Reactive Blue 19 | pH 4.0, temperature not specified in the publication | Irpex lacteus | |
| 224 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3,0, temperature not specified in the publication | Irpex lacteus | |
| 419 | - |
H2O2 | pH 3.0, temperature not specified in the publication | Irpex lacteus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.83 | - |
veratryl alcohol | pH 2.0, temperature not specified in the publication | Irpex lacteus | |
| 970 | - |
2,6-dimethoxyphenol | pH 3.0, temperature not specified in the publication | Irpex lacteus | |
| 1100 | - |
Reactive Black 5 | pH 3.0, temperature not specified in the publication | Irpex lacteus | |
| 5300 | - |
H2O2 | pH 3.0, temperature not specified in the publication | Irpex lacteus | |
| 5900 | - |
Reactive Blue 19 | pH 4.0, temperature not specified in the publication | Irpex lacteus | |
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