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Literature summary for 1.11.1.19 extracted from

  • Kim, S.J.; Shoda, M.
    Purification and characterization of a novel peroxidase from Geotrichum candidum dec 1 involved in decolorization of dyes (1999), Appl. Environ. Microbiol., 65, 1029-1035.
    View publication on PubMed
Search for more literature for 1.11.1.19

Inhibitors

Inhibitors Comment Organism Structure
H2O2 DyP activity for Reactive Blue 5 is inhibited when the H2O2 concentration exceeds 0.2 mM at a fixed DyP concentration of 0.6 nM. At 2.8 nM of DyP, its activity for 1,4-diamino-2-sodium anthraquinone sulfonate decreases sharply when the H2O2 concentration exceeds 0.1 mM Geotrichum candidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 H2O2 using Reactive Blue 5 as cosubstrate, at pH 3.2 and 30°C Geotrichum candidum
0.036
-
 H2O2 using 1-amino-4-methylamino-2-sodium anthraquinone sulfonate as cosubstrate, at pH 3.2 and 30°C Geotrichum candidum
0.054
-
 Reactive Blue 5 at pH 3.2 and 30°C Geotrichum candidum
0.084
-
 1-amino-4-methylamino-2-sodium anthraquinone sulfonate at pH 3.2 and 30°C Geotrichum candidum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no enhancement of the DyP activity by the addition of Mn2+ is observed Geotrichum candidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 1 * 55000, gel filtration Geotrichum candidum
60000
-
 SDS-PAGE Geotrichum candidum

Organism

Organism UniProt Comment Textmining
Geotrichum candidum
-
 strain Dec 1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein DyP is glycosylated with N-acetylglucosamine and mannose (17% (w/w)) Geotrichum candidum

Purification (Commentary)

Purification (Comment) Organism
Super Q 650M column chromatography and butyl Toyopearl column chromatography Geotrichum candidum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.28
-
 crude enzyme, in 25 mM citrate buffer (pH 3.2), at 30°C Geotrichum candidum
57
-
 after 210fold purification, in 25 mM citrate buffer (pH 3.2), at 30°C Geotrichum candidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-diamino-2-sodium anthraquinone sulfonate + H2O2 + H+
-
 Geotrichum candidum ?
-
 ?
1-amino-4-(3-amino-4-sodium-sulfonoanilino)-2-sodium anthraquinone sulfonate + H2O2 + H+
-
 Geotrichum candidum ?
-
 ?
1-amino-4-methylamino-2-sodium anthraquinone sulfonate + H2O2 + H+
-
 Geotrichum candidum ?
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Geotrichum candidum ?
-
 ?
guaiacol + H2O2 + H+
-
 Geotrichum candidum ?
-
 ?
additional information higher decolorizing activity is observed for anthraquinone dyes than for azo dyes. DyP does not oxidize veratryl alcohol Geotrichum candidum ?
-
 ?
Reactive Black 5 + H2O2 + H+
-
 Geotrichum candidum oxidized Reactive Black 5 + H2O
-
 ?
Reactive Blue 114 + H2O2 + H+
-
 Geotrichum candidum oxidized Reactive Blue 114 + H2O
-
 ?
Reactive Blue 182 + H2O2 + H+
-
 Geotrichum candidum oxidized Reactive Blue 182 + H2O
-
 ?
Reactive Blue 19 + H2O2 + H+
-
 Geotrichum candidum oxidized Reactive Blue 19 + H2O
-
 ?
Reactive Blue 5 + H2O2 + H+
-
 Geotrichum candidum oxidized Reactive Blue 5 + H2O
-
 ?
Reactive Red 33 + H2O2 + H+
-
 Geotrichum candidum oxidized Reactive Red 33 + H2O
-
 ?
Reactive Yellow 2 + H2O2 + H+
-
 Geotrichum candidum oxidized Reactive Yellow 2 + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, gel filtration Geotrichum candidum

Synonyms

Synonyms Comment Organism
DyP
-
 Geotrichum candidum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Geotrichum candidum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 35
-
 Geotrichum candidum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 DyP activity is restored at 30°C after treatment at 40 and 50°C for 11 h, DyP activity is stable after incubation at 50°C for 11 h. When DyP is incubated at 30 and 40°C for 14 days, the inactivation rates are 37 and 59%, respectively. When DyP is heated at 60°C for 3 h, 35% of the initial activity is lost Geotrichum candidum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
260
-
 Reactive Blue 5 at pH 3.2 and 30°C Geotrichum candidum
260
-
 H2O2 at pH 3.2 and 30°C Geotrichum candidum
270
-
 H2O2 using AQ2 as cosubstrate, at pH 3.2 and 30°C Geotrichum candidum
270
-
 1-amino-4-methylamino-2-sodium anthraquinone sulfonate at pH 3.2 and 30°C Geotrichum candidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 using 1-amino-4-methylamino-2-sodium anthraquinone sulfonate as substrate Geotrichum candidum
3.2
-
 using Reactive Blue 5, Reactive Blue 19, 1-amino-4-(3-amino-4-sodium-sulfonoanilino)-2-sodium anthraquinone sulfonate, 1,4-diamino-2-sodium anthraquinone sulfonate, Reactive Black 5, Reactive Red 33, or Reactive Yellow 2 as substrate Geotrichum candidum
4
-
 using Reactive Blue 182 or Reactive Blue 114 as substrate Geotrichum candidum

Cofactor

Cofactor Comment Organism Structure
heme
-
 Geotrichum candidum

pI Value

Organism Comment pI Value Maximum pI Value
Geotrichum candidum isoelectric focusing
-
 3.8

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3200
-
 1-amino-4-methylamino-2-sodium anthraquinone sulfonate at pH 3.2 and 30°C Geotrichum candidum
4800
-
 Reactive Blue 5 at pH 3.2 and 30°C Geotrichum candidum
7600
-
 H2O2 using AQ2 as cosubstrate, at pH 3.2 and 30°C Geotrichum candidum
10000
-
 H2O2 using Reactive Blue 5 as cosubstrate, at pH 3.2 and 30°C Geotrichum candidum