Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | substrate inhibition | Bjerkandera fumosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
2,7-diaminofluorene | absence of Mn2+, pH 4.5, temperature not specified in the publication | Bjerkandera fumosa | |
0.035 | - |
syringaldazine | presence of Mn2+, pH 7.0, temperature not specified in the publication | Bjerkandera fumosa | |
0.038 | - |
2,7-diaminofluorene | presence of Mn2+, pH not specified in the publication, temperature not specified in the publication | Bjerkandera fumosa | |
0.063 | - |
2,6-dimethoxyphenol | presence of Mn2+, pH 7.0, temperature not specified in the publication | Bjerkandera fumosa | |
0.066 | - |
syringaldazine | absence of Mn2+, pH 7.0, temperature not specified in the publication | Bjerkandera fumosa | |
0.304 | - |
veratryl alcohol | absence of Mn2+, pH 3.0, temperature not specified in the publication | Bjerkandera fumosa | |
0.345 | - |
2,6-dimethoxyphenol | absence of Mn2+, pH not specified in the publication, temperature not specified in the publication | Bjerkandera fumosa | |
0.48 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | absence of Mn2+, pH 3.0, temperature not specified in the publication | Bjerkandera fumosa | |
0.53 | - |
veratryl alcohol | presence of Mn2+, pH 4.5, temperature not specified in the publication | Bjerkandera fumosa | |
0.68 | - |
Mn2+ | pH not specified in the publication, temperature not specified in the publication | Bjerkandera fumosa | |
0.86 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | presence of Mn2+, pH 3.0, temperature not specified in the publication | Bjerkandera fumosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bjerkandera fumosa | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 2,6-dimethoxyphenol + 2 H2O2 | - |
Bjerkandera fumosa | coerulignone + 2 H2O | - |
? | |
2 Mn2+ + 2 H+ | - |
Bjerkandera fumosa | 2 Mn3+ + H2 | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Bjerkandera fumosa | ? + H2O | - |
? | |
2,7-diaminofluorene + H2O2 | during oxidation of 2,7-diaminofluorene, both with and without Mn2+, biphasic kinetics with apparent saturation in both micromolar and millimolar ranges are obtained | Bjerkandera fumosa | ? + H2O | - |
? | |
4-aminobenzoic acid + H2O2 | - |
Bjerkandera fumosa | ? + H2O | - |
? | |
anthracene + H2O2 | - |
Bjerkandera fumosa | 9,10-anthraquinone + H2O | - |
? | |
chrysene + H2O2 | - |
Bjerkandera fumosa | ? + H2O | - |
? | |
fluoranthene + H2O2 | - |
Bjerkandera fumosa | ? + H2O | - |
? | |
fluorene + H2O2 | - |
Bjerkandera fumosa | 9-fluorenone + H2O | - |
? | |
additional information | oxidation of substrates may occur in two ways, either directly by the enzyme or by diffusible chelated Mn3+ as an oxidizing agent | Bjerkandera fumosa | ? | - |
? | |
phenanthrene + H2O2 | - |
Bjerkandera fumosa | 9,10-phenanthrenequinone + H2O | - |
? | |
pyrene + H2O2 | - |
Bjerkandera fumosa | ? + H2O | - |
? | |
syringaldazine + H2O2 | - |
Bjerkandera fumosa | ? | - |
? | |
veratryl alcohol + H2O2 + H+ | - |
Bjerkandera fumosa | veratraldehyde + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
2,7-diaminofluorene | absence of Mn2+, pH 4.5, temperature not specified in the publication | Bjerkandera fumosa | |
0.22 | - |
syringaldazine | absence of Mn2+, pH 7.0, temperature not specified in the publication | Bjerkandera fumosa | |
0.83 | - |
2,7-diaminofluorene | presence of Mn2+, pH 7.0, temperature not specified in the publication | Bjerkandera fumosa | |
2.77 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | absence of Mn2+, pH 3.0, temperature not specified in the publication | Bjerkandera fumosa | |
3.9 | - |
Mn2+ | pH not specified in the publication, temperature not specified in the publication | Bjerkandera fumosa | |
6.1 | - |
syringaldazine | presence of Mn2+, pH 7.0, temperature not specified in the publication | Bjerkandera fumosa | |
6.2 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | presence of Mn2+, pH 3.0, temperature not specified in the publication | Bjerkandera fumosa | |
7.08 | - |
veratryl alcohol | absence of Mn2+, pH 3.0, temperature not specified in the publication | Bjerkandera fumosa | |
10.6 | - |
2,6-dimethoxyphenol | absence of Mn2+, pH not specified in the publication, temperature not specified in the publication | Bjerkandera fumosa | |
26.8 | - |
veratryl alcohol | presence of Mn2+, pH 4.5, temperature not specified in the publication | Bjerkandera fumosa | |
30 | - |
2,6-dimethoxyphenol | presence of Mn2+, pH not specified in the publication, temperature not specified in the publication | Bjerkandera fumosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
and 4.2, substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), presence of Mn2+ | Bjerkandera fumosa |
3 | - |
and 6.5, substrate veratryl alcohol, absence of Mn2+ | Bjerkandera fumosa |
3 | - |
substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), absence of Mn2+ | Bjerkandera fumosa |
4.2 | - |
and 3.0, substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), presence of Mn2+ | Bjerkandera fumosa |
4.5 | - |
and 7.0, substrate 2,7-diaminofluorene, absence of Mn2+ | Bjerkandera fumosa |
4.5 | - |
substrate veratryl alcohol, presence of Mn2+ | Bjerkandera fumosa |
5 | - |
and 6.5, substrate 2,7-diaminofluorene, presence of Mn2+ | Bjerkandera fumosa |
6.5 | - |
and 3.0, substrate veratryl alcohol, absence of Mn2+ | Bjerkandera fumosa |
6.5 | - |
and 3.0, substrate veratryl alcohol, presence of Mn2+ | Bjerkandera fumosa |
6.5 | - |
and 5.0, substrate 2,7-diaminofluorene, presence of Mn2+ | Bjerkandera fumosa |
7 | - |
and 4.5, substrate 2,7-diaminofluorene, absence of Mn2+ | Bjerkandera fumosa |
7 | - |
substrate syringaldazine, absence and presence of Mn2+ | Bjerkandera fumosa |
7 | - |
substrate syringaldazine, absence of Mn2+ | Bjerkandera fumosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
veratryl alcohol | absence of Mn2+, pH 3.0, temperature not specified in the publication | Bjerkandera fumosa | |
3.3 | - |
syringaldazine | absence of Mn2+, pH 7.0, temperature not specified in the publication | Bjerkandera fumosa | |
5.7 | - |
Mn2+ | pH not specified in the publication, temperature not specified in the publication | Bjerkandera fumosa | |
5.8 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | absence of Mn2+, pH 3.0, temperature not specified in the publication | Bjerkandera fumosa | |
7.2 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | presence of Mn2+, pH 3.0, temperature not specified in the publication | Bjerkandera fumosa | |
21.1 | - |
2,7-diaminofluorene | absence of Mn2+, pH 4.5, temperature not specified in the publication | Bjerkandera fumosa | |
21.8 | - |
2,7-diaminofluorene | presence of Mn2+, pH 7.0, temperature not specified in the publication | Bjerkandera fumosa | |
30.7 | - |
2,6-dimethoxyphenol | absence of Mn2+, pH not specified in the publication, temperature not specified in the publication | Bjerkandera fumosa | |
50.6 | - |
veratryl alcohol | presence of Mn2+, pH 4.5, temperature not specified in the publication | Bjerkandera fumosa | |
174.3 | - |
syringaldazine | presence of Mn2+, pH 7.0, temperature not specified in the publication | Bjerkandera fumosa | |
476.2 | - |
2,6-dimethoxyphenol | presence of Mn2+, pH not specified in the publication, temperature not specified in the publication | Bjerkandera fumosa |