Protein Variants | Comment | Organism |
---|---|---|
H232F | not involved in long-range electron transfer | Pleurotus eryngii |
P76H | not involved in long-range electron transfer | Pleurotus eryngii |
W164H | no enzymic activity with veratryl alcohol or Reactive Black 5 | Pleurotus eryngii |
W164S | no enzymic activity with veratryl alcohol or Reactive Black 5 | Pleurotus eryngii |
W164S/P76H | no enzymic activity with veratryl alcohol or Reactive Black 5 | Pleurotus eryngii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
Reactive Black 5 | wild-tpye, pH 3.5 | Pleurotus eryngii | |
0.0031 | - |
Reactive Black 5 | mutant P76H, pH 3.5 | Pleurotus eryngii | |
0.0036 | - |
Reactive Black 5 | mutant H232F, pH 3.5 | Pleurotus eryngii | |
0.11 | - |
Mn2+ | mutant W164S, pH 5.0 | Pleurotus eryngii | |
0.126 | - |
Mn2+ | mutant W164H, pH 5.0 | Pleurotus eryngii | |
0.189 | - |
Mn2+ | wild-tpye, pH 5.0 | Pleurotus eryngii | |
0.218 | - |
Mn2+ | mutant H232F, pH 5.0 | Pleurotus eryngii | |
0.262 | - |
Mn2+ | mutant P76H, pH 5.0 | Pleurotus eryngii | |
0.351 | - |
Mn2+ | mutant W164S/P76H, pH 5.0 | Pleurotus eryngii | |
2.41 | - |
veratryl alcohol | mutant P76H, pH 3.0 | Pleurotus eryngii | |
2.75 | - |
veratryl alcohol | wild-tpye, pH 3.0 | Pleurotus eryngii | |
3.58 | - |
veratryl alcohol | mutant H232F, pH 3.0 | Pleurotus eryngii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | - |
isoenzyme VPL2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H2O2 | - |
Pleurotus eryngii | Mn3+ + H2O | - |
? | |
Reactive Black 5 + H2O2 | - |
Pleurotus eryngii | oxidized Reactive Black 5 + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | - |
Pleurotus eryngii | veratraldehyde + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
Reactive Black 5 | mutant H232F, pH 3.5 | Pleurotus eryngii | |
5 | - |
Reactive Black 5 | wild-type, pH 3.5 | Pleurotus eryngii | |
5 | - |
Reactive Black 5 | 0.0 M NaCl, 55.6 IgG relative content, 5.5% relative DNase activity | Pleurotus eryngii | |
6 | - |
Reactive Black 5 | mutant P76H, pH 3.5 | Pleurotus eryngii | |
8 | - |
veratryl alcohol | wild-type, pH 3.0 | Pleurotus eryngii | |
8 | - |
veratryl alcohol | H196A mutant, 30°C, pH 6.5 | Pleurotus eryngii | |
11 | - |
veratryl alcohol | mutant P76H, pH 3.0 | Pleurotus eryngii | |
14 | - |
veratryl alcohol | mutant H232F, pH 3.0 | Pleurotus eryngii | |
207 | - |
Mn2+ | mutant W164S, pH 5.0 | Pleurotus eryngii | |
247 | - |
Mn2+ | mutant W164S/P76H, pH 5.0 | Pleurotus eryngii | |
291 | - |
Mn2+ | mutant P76H, pH 5.0 | Pleurotus eryngii | |
298 | - |
Mn2+ | wild-type, pH 5.0 | Pleurotus eryngii | |
298 | - |
Mn2+ | 0.000083 1/sec/mg, incubates 30 min at 37°C, pH 9.0, spectrophotometrically measured at 415 nm | Pleurotus eryngii | |
308 | - |
Mn2+ | mutant H232F, pH 5.0 | Pleurotus eryngii | |
320 | - |
Mn2+ | mutant W164H, pH 5.0 | Pleurotus eryngii |