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Literature summary for 1.11.1.16 extracted from

  • Perez-Boada, M.; Ruiz-Duenas, F.J.; Pogni, R.; Basosi, R.; Choinowski, T.; Martinez, M.J.; Piontek, K.; Martinez, A.T.
    Versatile peroxidase oxidation of high redox potential aromatic compounds: site-directed mutagenesis, spectroscopic and crystallographic investigation of three long-range electron transfer pathways (2005), J. Mol. Biol., 354, 385-402.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
H232F not involved in long-range electron transfer Pleurotus eryngii
P76H not involved in long-range electron transfer Pleurotus eryngii
W164H no enzymic activity with veratryl alcohol or Reactive Black 5 Pleurotus eryngii
W164S no enzymic activity with veratryl alcohol or Reactive Black 5 Pleurotus eryngii
W164S/P76H no enzymic activity with veratryl alcohol or Reactive Black 5 Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
Reactive Black 5 wild-tpye, pH 3.5 Pleurotus eryngii
0.0031
-
Reactive Black 5 mutant P76H, pH 3.5 Pleurotus eryngii
0.0036
-
Reactive Black 5 mutant H232F, pH 3.5 Pleurotus eryngii
0.11
-
Mn2+ mutant W164S, pH 5.0 Pleurotus eryngii
0.126
-
Mn2+ mutant W164H, pH 5.0 Pleurotus eryngii
0.189
-
Mn2+ wild-tpye, pH 5.0 Pleurotus eryngii
0.218
-
Mn2+ mutant H232F, pH 5.0 Pleurotus eryngii
0.262
-
Mn2+ mutant P76H, pH 5.0 Pleurotus eryngii
0.351
-
Mn2+ mutant W164S/P76H, pH 5.0 Pleurotus eryngii
2.41
-
veratryl alcohol mutant P76H, pH 3.0 Pleurotus eryngii
2.75
-
veratryl alcohol wild-tpye, pH 3.0 Pleurotus eryngii
3.58
-
veratryl alcohol mutant H232F, pH 3.0 Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii
-
isoenzyme VPL2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Mn2+ + H2O2
-
Pleurotus eryngii Mn3+ + H2O
-
?
Reactive Black 5 + H2O2
-
Pleurotus eryngii oxidized Reactive Black 5 + H2O
-
?
veratryl alcohol + H2O2 + H+
-
Pleurotus eryngii veratraldehyde + H2O
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.7
-
Reactive Black 5 mutant H232F, pH 3.5 Pleurotus eryngii
5
-
Reactive Black 5 wild-type, pH 3.5 Pleurotus eryngii
5
-
Reactive Black 5 0.0 M NaCl, 55.6 IgG relative content, 5.5% relative DNase activity Pleurotus eryngii
6
-
Reactive Black 5 mutant P76H, pH 3.5 Pleurotus eryngii
8
-
veratryl alcohol wild-type, pH 3.0 Pleurotus eryngii
8
-
veratryl alcohol H196A mutant, 30°C, pH 6.5 Pleurotus eryngii
11
-
veratryl alcohol mutant P76H, pH 3.0 Pleurotus eryngii
14
-
veratryl alcohol mutant H232F, pH 3.0 Pleurotus eryngii
207
-
Mn2+ mutant W164S, pH 5.0 Pleurotus eryngii
247
-
Mn2+ mutant W164S/P76H, pH 5.0 Pleurotus eryngii
291
-
Mn2+ mutant P76H, pH 5.0 Pleurotus eryngii
298
-
Mn2+ wild-type, pH 5.0 Pleurotus eryngii
298
-
Mn2+ 0.000083 1/sec/mg, incubates 30 min at 37°C, pH 9.0, spectrophotometrically measured at 415 nm Pleurotus eryngii
308
-
Mn2+ mutant H232F, pH 5.0 Pleurotus eryngii
320
-
Mn2+ mutant W164H, pH 5.0 Pleurotus eryngii