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Literature summary for 1.11.1.14 extracted from

  • Miki, Y.; Morales, M.; Ruiz-Duenas, F.J.; Martinez, M.J.; Wariishi, H.; Martinez, A.T.
    Escherichia coli expression and in vitro activation of a unique ligninolytic peroxidase that has a catalytic tyrosine residue (2009), Protein Expr. Purif., 68, 208-214.
    View publication on PubMed
Search for more literature for 1.11.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells, recombinant LiP protein accumulates in inclusion bodies as an inactive form Trametopsis cervina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.79
-
 veratryl alcohol recombinant enzyme without veratryl alcohol-H2O2 pretreatment, in 20 mM sodium succinate buffer (pH 3.0) Trametopsis cervina
3.24
-
 veratryl alcohol recombinant enzyme with veratryl alcohol-H2O2 pretreatment, in 20 mM sodium succinate buffer (pH 3.0) Trametopsis cervina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
veratryl alcohol + H2O2 Trametopsis cervina
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trametopsis cervina Q60FD2
-
-

Purification (Commentary)

Purification (Comment) Organism
Resource Q column chromatography, gel filtration Trametopsis cervina

Renatured (Commentary)

Renatured (Comment) Organism
the optimal refolding solution contains of 0.4 M urea, 5 mM CaCl2, 0.0005 mM hemin, and 0.6 mM oxidized glutathione in Tris-HCl buffer, at pH 9.5 Trametopsis cervina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-dimethoxybenzene + H2O2
-
 Trametopsis cervina ?
-
 ?
ferrocytochrome c + H2O2
-
 Trametopsis cervina ?
-
 ?
reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
-
 Trametopsis cervina oxidized 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O
-
 ?
veratryl alcohol + H2O2
-
 Trametopsis cervina ?
-
 ?

Synonyms

Synonyms Comment Organism
lignin peroxidase
-
 Trametopsis cervina
LIP
-
 Trametopsis cervina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the recombinant enzyme is fully stable up to 30°C but unstable at 37°C, and is completely inactivated after 30 min at 60°C Trametopsis cervina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.3
-
 veratryl alcohol recombinant enzyme without veratryl alcohol-H2O2 pretreatment, in 20 mM sodium succinate buffer (pH 3.0) Trametopsis cervina
17.7
-
 veratryl alcohol recombinant enzyme with veratryl alcohol-H2O2 pretreatment, in 20 mM sodium succinate buffer (pH 3.0) Trametopsis cervina

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 the recombiannt enzyme is stable from pH 3.5 to neutral pH, but unstable over pH 7.0 and below pH 3.0 Trametopsis cervina