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Literature summary for 1.11.1.13 extracted from

  • Fernandez-Fueyo, E.; Ruiz-Duenas, F.J.; Martinez, A.T.
    Engineering a fungal peroxidase that degrades lignin at very acidic pH (2014), Biotechnol. Biofuels, 7, 114 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Gelatoporia subvermispora

Protein Variants

Protein Variants Comment Organism
S168W mutation introduced to confer Vversatile peroxidase-type activity, EC 1.11.1.16, on aromatic substrates and dyes. Variant conserves the high catalytic efficiency of isoform MnP6 oxidizing Mn2+ and gains the ability to oxidize veratryl alcohol as well as reactive black 5 Gelatoporia subvermispora
T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G mutation introduced to confer Vversatile peroxidase-type activity, EC 1.11.1.16, on aromatic substrates and dyes. Variant conserves the high catalytic efficiency of isoform MnP6 oxidizing Mn2+ and gains the ability to oxidize veratryl alcohol as well as reactive black 5 Gelatoporia subvermispora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0054
-
Reactive Black 5 mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 Gelatoporia subvermispora
0.0085
-
Mn(II) mutant S168W, 25°C, pH 5.0 Gelatoporia subvermispora
0.0087
-
Mn(II) wild-type, 25°C, pH 5.0 Gelatoporia subvermispora
0.011
-
Mn(II) mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 Gelatoporia subvermispora
0.0126
-
Reactive Black 5 mutant S168W, 25°C, pH 5.0 Gelatoporia subvermispora
0.06
-
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) mutant S168W, 25°C, pH 5.0 Gelatoporia subvermispora
2.74
-
veratryl alcohol mutant S168W, 25°C, pH 5.0 Gelatoporia subvermispora
21.6
-
veratryl alcohol mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 Gelatoporia subvermispora

Organism

Organism UniProt Comment Textmining
Gelatoporia subvermispora M2REM6
-
-
Gelatoporia subvermispora B M2REM6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 Mn(II) + 2 H+ + H2O2
-
Gelatoporia subvermispora 2 Mn(III) + 2 H2O
-
?
2 Mn(II) + 2 H+ + H2O2
-
Gelatoporia subvermispora B 2 Mn(III) + 2 H2O
-
?
2 veratryl alcohol + H2O2 no substrate of wild-type Gelatoporia subvermispora 2 veratraldehyde + H2O
-
?
2 veratryl alcohol + H2O2 no substrate of wild-type Gelatoporia subvermispora B 2 veratraldehyde + H2O
-
?
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 no substrate of wild-type Gelatoporia subvermispora ?
-
?
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 no substrate of wild-type Gelatoporia subvermispora B ?
-
?
Reactive Black 5 + H2O2 no substrate of wild-type Gelatoporia subvermispora ? + H2O
-
?
Reactive Black 5 + H2O2 no substrate of wild-type Gelatoporia subvermispora B ? + H2O
-
?

Synonyms

Synonyms Comment Organism
MnP6
-
Gelatoporia subvermispora

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.54
-
veratryl alcohol mutant S168W, 25°C, pH 5.0 Gelatoporia subvermispora
1.4
-
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) mutant S168W, 25°C, pH 5.0 Gelatoporia subvermispora
1.69
-
veratryl alcohol mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 Gelatoporia subvermispora
7
-
Reactive Black 5 mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 Gelatoporia subvermispora
7.8
-
Reactive Black 5 mutant S168W, 25°C, pH 5.0 Gelatoporia subvermispora
60.5
-
Mn(II) mutant S168W, 25°C, pH 5.0 Gelatoporia subvermispora
66.9
-
Mn(II) mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 Gelatoporia subvermispora
83
-
Mn(II) wild-type, 25°C, pH 5.0 Gelatoporia subvermispora

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.078
-
veratryl alcohol mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 Gelatoporia subvermispora
0.197
-
veratryl alcohol mutant S168W, 25°C, pH 5.0 Gelatoporia subvermispora
23
-
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) mutant S168W, 25°C, pH 5.0 Gelatoporia subvermispora
619
-
Reactive Black 5 mutant S168W, 25°C, pH 5.0 Gelatoporia subvermispora
1310
-
Reactive Black 5 mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 Gelatoporia subvermispora
6060
-
Mn(II) mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 Gelatoporia subvermispora
7110
-
Mn(II) mutant S168W, 25°C, pH 5.0 Gelatoporia subvermispora
9540
-
Mn(II) wild-type, 25°C, pH 5.0 Gelatoporia subvermispora