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Literature summary for 1.11.1.13 extracted from

  • Petruccioli, M.; Frasconi, M.; Quaratino, D.; Covino, S.; Favero, G.; Mazzei, F.; Federici, F.; DAnnibale, A.
    Kinetic and redox properties of MnP II, a major manganese peroxidase isoenzyme from Panus tigrinus CBS 577.79 (2009), J. Biol. Inorg. Chem., 14, 1153-1163.
    View publication on PubMed
Search for more literature for 1.11.1.13

Activating Compound

Activating Compound Comment Organism Structure
diphosphate diphosphate proves to be the worst chelator, leading to a specific activity about 4.6% of that obtained with tartrate Lentinus tigrinus
Lactate lactate serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 48.4% of that obtained with tartrate Lentinus tigrinus
malonate the highest stimulation of 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate, malonate also serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 54.8% of that obtained with tartrate Lentinus tigrinus
oxalate oxalate serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 64.7% of that obtained with tartrate Lentinus tigrinus
Tartrate the highest rate of Mn3+ formation is obtained with 10 mM tartrate Lentinus tigrinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 H2O2
-
 Lentinus tigrinus
0.016
-
 H2O2 pH 5.5, 45°C Lentinus tigrinus
0.0713
-
 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid)
-
 Lentinus tigrinus
0.0713
-
 2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus
0.076
-
 2,6-dimethoxyphenol
-
 Lentinus tigrinus
0.076
-
 2,6-dimethoxyphenol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
0.124
-
 Mn2+
-
 Lentinus tigrinus
0.124
-
 Mn2+ pH 5.5, 45°C Lentinus tigrinus
0.196
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
 Lentinus tigrinus
0.196
-
 2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
0.213
-
 guaiacol
-
 Lentinus tigrinus
0.213
-
 guaiacol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
0.293
-
 pyrogallol
-
 Lentinus tigrinus
0.293
-
 pyrogallol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
0.56
-
 veratryl alcohol
-
 Lentinus tigrinus
0.56
-
 veratryl alcohol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
2.3
-
 guaiacol
-
 Lentinus tigrinus
2.3
-
 guaiacol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus
3.74
-
 pyrogallol
-
 Lentinus tigrinus
3.74
-
 pyrogallol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus
6.954
-
 2,6-dimethoxyphenol
-
 Lentinus tigrinus
6.954
-
 2,6-dimethoxyphenol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus

Metals/Ions

Metals/Ions Comment Organism Structure
Manganese
-
 Lentinus tigrinus
Mn2+ veratryl alcohol oxidation requires the simultaneous presence of H2O2 and Mn2+ Lentinus tigrinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50500
-
 gel filtration Lentinus tigrinus
50500
-
 determined by gel filtration and SDS-PAGE Lentinus tigrinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Mn2+ + H+ + H2O2 Lentinus tigrinus
-
 Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 Lentinus tigrinus CBS 577.79
-
 Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 Lentinus tigrinus 577.79
-
 Mn3+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Lentinus tigrinus
-
-
-
Lentinus tigrinus 577.79
-
-
-
Lentinus tigrinus CBS 577.79
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylation of about 5.3% of the high-mannose type Lentinus tigrinus
glycoprotein the enzyme displays an extent of N-glycosylation of about 5.3% of the high-mannose type Lentinus tigrinus

Purification (Commentary)

Purification (Comment) Organism
purification scheme involves ultrafiltration, affinity chromatography on concanavalin-A Sepharose and gel filtration Lentinus tigrinus
ultrafiltration, Concanavalin-A Sepharose column chromatography and Superdex 75 gel filtration Lentinus tigrinus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40
-
 purification step culture supernatant Lentinus tigrinus
40
-
 culture supernatant, pH 5.5, 45°C Lentinus tigrinus
41
-
 purification step ultrafiltration Lentinus tigrinus
169
-
 purification step chromatography on a concanavalin-A Sepharose column Lentinus tigrinus
288
-
 purification step gel filtration Lentinus tigrinus
288
-
 after 7.2fold purification, pH 5.5, 45°C Lentinus tigrinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 + H+
-
 Lentinus tigrinus ?
-
 r
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ?
-
 Lentinus tigrinus ?
-
 ?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ?
-
 Lentinus tigrinus CBS 577.79 ?
-
 ?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ?
-
 Lentinus tigrinus 577.79 ?
-
 ?
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid + H2O2
-
 Lentinus tigrinus ?
-
 ?
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid + H2O2 + Mn2+
-
 Lentinus tigrinus ?
-
 ?
2,6-dimethoxyphenol + H2O2 the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate Lentinus tigrinus ?
-
 ?
2,6-dimethoxyphenol + H2O2 the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate Lentinus tigrinus CBS 577.79 ?
-
 ?
2,6-dimethoxyphenol + H2O2 the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate Lentinus tigrinus 577.79 ?
-
 ?
2,6-dimethoxyphenol + H2O2 + Mn2+
-
 Lentinus tigrinus ?
-
 ?
2,6-dimethoxyphenol + Mn2+ + ?
-
 Lentinus tigrinus ?
-
 ?
guaiacol + H2O2
-
 Lentinus tigrinus ?
-
 ?
guaiacol + H2O2
-
 Lentinus tigrinus CBS 577.79 ?
-
 ?
guaiacol + H2O2
-
 Lentinus tigrinus 577.79 ?
-
 ?
guaiacol + H2O2 + Mn2+
-
 Lentinus tigrinus ?
-
 ?
guaiacol + Mn2+ + ?
-
 Lentinus tigrinus ?
-
 ?
Mn2+ + H+ + H2O2
-
 Lentinus tigrinus Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2
-
 Lentinus tigrinus CBS 577.79 Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2
-
 Lentinus tigrinus 577.79 Mn3+ + H2O
-
 ?
pyrogallol + H2O2
-
 Lentinus tigrinus ?
-
 ?
pyrogallol + H2O2
-
 Lentinus tigrinus CBS 577.79 ?
-
 ?
pyrogallol + H2O2
-
 Lentinus tigrinus 577.79 ?
-
 ?
pyrogallol + H2O2 + Mn2+
-
 Lentinus tigrinus ?
-
 ?
pyrogallol + Mn2+ + ?
-
 Lentinus tigrinus ?
-
 ?
veratryl alcohol + H2O2 + Mn2+ veratryl alcohol oxidation requires the simultaneous presence of H2O2 and Mn2+ Lentinus tigrinus ?
-
 ?
veratryl alcohol + Mn2+ + ?
-
 Lentinus tigrinus ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Lentinus tigrinus
monomer 1 * 50500, SDS-PAGE Lentinus tigrinus

Synonyms

Synonyms Comment Organism
manganese peroxidase
-
 Lentinus tigrinus
Mn2+: hydrogen peroxide oxidoreductase
-
 Lentinus tigrinus
Mn2+:hydrogen peroxide oxidoreductase
-
 Lentinus tigrinus
MnP II
-
 Lentinus tigrinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Lentinus tigrinus
45
-
 optimum temperature for the oxidation of Mn2+ and DMOP Lentinus tigrinus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 the enzyme shows relative activities of 34, 50, and 47% at 10, 40, and 60°C, respectively Lentinus tigrinus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 the enzyme is rather unstable at 50, 60, and 70°C, resulting in half-lives of 660, 105, and 41 s, respectively Lentinus tigrinus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
 veratryl alcohol
-
 Lentinus tigrinus
0.45
-
 veratryl alcohol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
0.74
-
 guaiacol
-
 Lentinus tigrinus
0.74
-
 guaiacol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus
1.87
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) Mn2+
-
 Lentinus tigrinus
1.87
-
 2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus
5.67
-
 2,6-dimethoxyphenol
-
 Lentinus tigrinus
5.67
-
 2,6-dimethoxyphenol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus
8.92
-
 guaiacol
-
 Lentinus tigrinus
8.92
-
 guaiacol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
9.98
-
 pyrogallol
-
 Lentinus tigrinus
9.98
-
 pyrogallol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus
20.2
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
 Lentinus tigrinus
20.2
-
 2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
20.95
-
 pyrogallol
-
 Lentinus tigrinus
20.95
-
 pyrogallol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
26.26
-
 2,6-dimethoxyphenol
-
 Lentinus tigrinus
26.26
-
 2,6-dimethoxyphenol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
121
-
 H2O2
-
 Lentinus tigrinus
121
-
 H2O2 with 1.5 mM Mn2+ as cosubstrate, pH 5.5, 45°C Lentinus tigrinus
242.4
-
 Mn2+
-
 Lentinus tigrinus
242.4
-
 Mn2+ with 0.2 mM H2O2 as cosubstrate, pH 5.5, 45°C Lentinus tigrinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 optimal pH for the Mn-dependent oxidation of veratryl alcohol Lentinus tigrinus
3
-
 optimum pH for the Mn2+-dependent oxidation of veratryl alehyde Lentinus tigrinus
3.5 4.5 pH optimum for Mn2+-independent DMOP oxidation Lentinus tigrinus
4 4.5 pH optimum for Mn2+-dependent DMOP oxidation Lentinus tigrinus
5.5
-
-
 Lentinus tigrinus

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6.5 Mn2+ oxidation shows an optimum in the range 5.5-5.75 and negligible activity at pH values below 3.5 and above 6.5 Lentinus tigrinus
3.5 4.5 optima for Mn2+-independent 2,6-dimethoxyphenol oxidation Lentinus tigrinus
4 4.5 optima for Mn2+-dependent 2,6-dimethoxyphenol oxidation Lentinus tigrinus
5.5 5.8 the pH-activity profile for Mn2+ oxidation shows an optimum in the range 5.5 - 5.75 and negligible activity at pHs below 3.5 and above 6.5 Lentinus tigrinus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 5.5 the enzyme shows a half-life of 8.8 h at pH 3.5, the half-lives of MnP activity at pH 4.5, 5.0, and 5.5 are 89.5, 99, and 101 h, respectively Lentinus tigrinus

Cofactor

Cofactor Comment Organism Structure
heme the heme content is 0.96 mol per mole of the enzyme Lentinus tigrinus

pI Value

Organism Comment pI Value Maximum pI Value
Lentinus tigrinus
-
-
 4.07
Lentinus tigrinus isoelectric focusing
-
 4.1