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Literature summary for 1.11.1.13 extracted from
- Implication of Dichomitus squalens manganese-dependent peroxidase in dye decolorization and cooperation of the enzyme with laccase (2008), Folia Microbiol. (Praha), 53, 479-485.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
2,6-dimethoxyphenol | apparent Km, 216 microM/l, chromatographic MnP form 2 | Dichomitus squalens |  |
| additional information | - |
Mn2+ | apparent Km, 24 microM/l, chromatographic MnP form 1 | Dichomitus squalens | |
| additional information | - |
2,6-dimethoxyphenol | apparent Km, 417 microM/l, chromatographic MnP form 1 | Dichomitus squalens | |
| additional information | - |
Mn2+ | apparent Km, 44 microM/l, chromatographic MnP form 2 | Dichomitus squalens | |
| additional information | - |
Mn2+ | apparent Km, 47 microM/l, chromatographic MnP form 3 | Dichomitus squalens | |
| additional information | - |
2,6-dimethoxyphenol | apparent Km, 516 microM/l, chromatographic MnP form 3 | Dichomitus squalens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Manganese | - |
Dichomitus squalens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
determined by SDS-PAGE, chromatographic MnP form 1, 2 and 3 | Dichomitus squalens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Mn2+ + H+ + H2O2 | Dichomitus squalens | - |
Mn3+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dichomitus squalens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| extract is loaded onto a HiPREP26/10 desalting column followd by chromatography on a DEAE-Sepharose and a MonoQ HR5/5 column, finally the active fractions are applied to CIM QA Disks | Dichomitus squalens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 185 | - |
purification step crude medium | Dichomitus squalens |
| 733 | - |
purification step DEAE Sepharose | Dichomitus squalens |
| 870 | - |
purification step Mono Q1 | Dichomitus squalens |
| 1230 | - |
purification step CIM QA disk 3, chromatographic MnP form 3, CIM3 | Dichomitus squalens |
| 2250 | - |
purification step Mono Q2 | Dichomitus squalens |
| 2480 | - |
purification step CIM QA disk 1, chromatographic MnP form 1, CIM1 | Dichomitus squalens |
| 3430 | - |
purification step CIM QA disk 2, chromatographic MnP form 2, CIM2 | Dichomitus squalens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,6-dimethoxyphenol + H2O2 | - |
Dichomitus squalens | ? | - |
? | |
| Mn2+ + H+ + H2O2 | - |
Dichomitus squalens | Mn3+ + H2O | - |
? | |
| additional information | dye decolorization | Dichomitus squalens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| manganese-dependent peroxidase | - |
Dichomitus squalens |
| MnP | - |
Dichomitus squalens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
pH optimum for Mn2+ oxidation, chromatographic MnP form 1, 2 and 3 | Dichomitus squalens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Dichomitus squalens | chromatographic MnP form 1 and 2 | - |
3.5 |
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