Cloned (Comment) | Organism |
---|---|
three differentexpression vectors are constructed: pZBMNP contains the native Phanerochaete chrysosporium fungal secretion signal, palphaAMNP contains an alpha-factor secretion signal derived from Saccharomyces cerevisiae, and pZBIMNP has no secretion signal and is used for intracellular expression | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
H2O2 | recombinant enzyme | Phanerodontia chrysosporium | |
0.058 | - |
H2O2 | wild-type enzyme | Phanerodontia chrysosporium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE | Phanerodontia chrysosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant enzyme produced by ZBMNP16 is hyperglycosylated | Phanerodontia chrysosporium |
Purification (Comment) | Organism |
---|---|
- |
Phanerodontia chrysosporium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dimethoxyphenol + H2O2 | - |
Phanerodontia chrysosporium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Phanerodontia chrysosporium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
pH 4.5, 3 min, wild-type enzyme loses about 75% of the initial activity, 50% loss of activity after 3 min in presence of 1 mM ferric chloride. pH 4.5, 2 min, recombinant enzyme loses about 90% of the initial activity, about 45% loss of activity in presence of 1 mM ferric chloride | Phanerodontia chrysosporium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
168 | - |
H2O2 | recombinant enzyme | Phanerodontia chrysosporium | |
237 | - |
H2O2 | wild-type enzyme | Phanerodontia chrysosporium |