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Literature summary for 1.11.1.13 extracted from

  • Sarkar, S.; Martinez, A.T.; Martinez, M.J.
    Biochemical and molecular characterization of a manganese peroxidase isoenzyme from Pleurotus ostreatus (1997), Biochim. Biophys. Acta, 1339, 23-30.
    View publication on PubMed
Search for more literature for 1.11.1.13

Activating Compound

Activating Compound Comment Organism Structure
additional information
-
 Phanerodontia chrysosporium
additional information strong increase of MnP levels by growth in presence of peptone, compared with glucose-ammonium tartrate medium Pleurotus ostreatus
additional information strong increase of MnP levels by growth in presence of peptone, compared with glucose-ammonium tartrate medium Pleurotus eryngii

Application

Application Comment Organism
nutrition biotechnological applications related to animal feeding Pleurotus ostreatus
nutrition biotechnological applications related to animal feeding Pleurotus eryngii
paper production
-
 Phanerodontia chrysosporium
paper production preferential degradation of lignin in wheat straw, an important property for biotechnological applications related to pulp and paper industry Pleurotus ostreatus
paper production preferential degradation of lignin in wheat straw, an important property for biotechnological applications related to pulp and paper industry Pleurotus eryngii

Cloned(Commentary)

Cloned (Comment) Organism
isoenzymes are encoded by different genes Pleurotus ostreatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 H2O2 0.1 mM 2,6-dimethoxyphenol Pleurotus ostreatus
0.01
-
 Mn2+ 0.1 mM 2,6-dimethoxyphenol, 0.1 mM H2O2 Pleurotus ostreatus
0.011
-
 2,6-dimethoxyphenol 0.1 mM Mn2+, 0.1 mM H2O2 Pleurotus ostreatus
0.015
-
 H2O2 0.1 mM Mn2+ Pleurotus ostreatus
0.018
-
 Mn2+ 0.1 mM H2O2 Pleurotus ostreatus
0.04
-
 NADH
-
 Pleurotus ostreatus
0.95
-
 2,6-dimethoxyphenol 0.1 mM H2O2 Pleurotus ostreatus
4.1
-
 veratryl alcohol 0.1 mM H2O2 Pleurotus ostreatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Pleurotus ostreatus
43000
-
 x * 43000, SDS-PAGE Pleurotus eryngii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Mn2+ + H+ + H2O2 Pleurotus ostreatus involved in lignin-degradation Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 Phanerodontia chrysosporium involved in lignin-degradation Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 Pleurotus eryngii involved in lignin-degradation Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 Pleurotus ostreatus preferential degradation of lignin in wheat straw Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 Pleurotus eryngii preferential degradation of lignin in wheat straw Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 Pleurotus ostreatus CBS 411.71 involved in lignin-degradation Mn3+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Lentinus tigrinus
-
-
-
Phanerodontia chrysosporium
-
 strain ATCC 24725
-
Pleurotus eryngii
-
 white rot fungus, strain CBS 613.91, 2 isoenzymes: MnP1 and MnP2
-
Pleurotus ostreatus
-
-
-
Pleurotus ostreatus
-
 importance of media composition in the production of different isoenzymes
-
Pleurotus ostreatus
-
 white rot basidomycete, 3 isoenzymes: MnP1, MnP2 and MnP3
-
Pleurotus ostreatus CBS 411.71
-
-
-
Rigidoporus microporus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Phanerodontia chrysosporium
glycoprotein 5% carbohydrate content Pleurotus ostreatus
glycoprotein 5% carbohydrate content Pleurotus eryngii

Purification (Commentary)

Purification (Comment) Organism
35.8fold purification Pleurotus ostreatus
MnP1 and MnP2 Pleurotus eryngii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
221
-
-
 Pleurotus ostreatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dimethoxyphenol + H2O2
-
 Pleurotus ostreatus ?
-
 ?
Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2
-
 Lentinus tigrinus Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2
-
 Pleurotus eryngii Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2
-
 Rigidoporus microporus Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 oxidizes Mn2+ as the best substrate Pleurotus ostreatus Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 involved in lignin-degradation Pleurotus ostreatus Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 involved in lignin-degradation Phanerodontia chrysosporium Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 involved in lignin-degradation Pleurotus eryngii Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 preferential degradation of lignin in wheat straw Pleurotus ostreatus Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 preferential degradation of lignin in wheat straw Pleurotus eryngii Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 oxidizes Mn2+ as the best substrate Pleurotus ostreatus CBS 411.71 Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 involved in lignin-degradation Pleurotus ostreatus CBS 411.71 Mn3+ + H2O
-
 ?
additional information in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Pleurotus ostreatus ?
-
 ?
additional information in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Pleurotus eryngii ?
-
 ?
additional information Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol Pleurotus ostreatus ?
-
 ?
additional information Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol Phanerodontia chrysosporium ?
-
 ?
additional information Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol Pleurotus eryngii ?
-
 ?
additional information Mn2+-independent oxidase activity on NAD(P)H Pleurotus ostreatus ?
-
 ?
additional information Mn2+-independent oxidase activity on NAD(P)H Phanerodontia chrysosporium ?
-
 ?
additional information Mn2+-independent oxidase activity on NAD(P)H Pleurotus eryngii ?
-
 ?
additional information in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Pleurotus ostreatus CBS 411.71 ?
-
 ?
additional information Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol Pleurotus ostreatus CBS 411.71 ?
-
 ?
additional information Mn2+-independent oxidase activity on NAD(P)H Pleurotus ostreatus CBS 411.71 ?
-
 ?
veratryl alcohol + H2O2
-
 Pleurotus ostreatus ? + H2O
-
 ?

Subunits

Subunits Comment Organism
?
-
 Phanerodontia chrysosporium
? x * 43000, SDS-PAGE Pleurotus ostreatus
? x * 43000, SDS-PAGE Pleurotus eryngii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
-
 Phanerodontia chrysosporium
additional information
-
-
 Pleurotus eryngii
additional information
-
 pI: 3.8 Pleurotus ostreatus
3
-
 oxidation of 2,6-dimethoxyphenol and veratryl alcohol in absence of Mn2+ Pleurotus ostreatus
3
-
 oxidation of 2,6-dimethoxyphenol and veratryl alcohol in absence of Mn2+ Pleurotus eryngii
3.5
-
 oxidation of NAD(P)H Pleurotus ostreatus
3.5
-
 oxidation of NAD(P)H Pleurotus eryngii
4.5
-
-
 Phanerodontia chrysosporium
4.5
-
 oxidation of 2,6-dimethoxyphenol in presence of Mn2+ Pleurotus ostreatus
5
-
-
 Phanerodontia chrysosporium
5
-
 oxidation of Mn2+ Pleurotus ostreatus
5
-
 oxidation of Mn2+ Pleurotus eryngii