Any feedback?
Literature summary for 1.11.1.13 extracted from
- Characteristics and N-terminal amino acid sequence of a manganese peroxidase purified from Lentinula edodes cultures grown on a commercial wood substrate (1990), Appl. Microbiol. Biotechnol., 33, 359-365.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | stimulates by chelating and stabilizing Mn3+ | Lentinula edodes |  |
| dithiothreitol | greatly enhances the oxidation of verartryl alcohol, lignin-model compounds and lignin | Lentinula edodes | |
| glutathione | greatly enhances the oxidation of verartryl alcohol, lignin-model compounds and lignin | Lentinula edodes | |
| H2O2 | H2O2-dependent | Lentinula edodes | |
| L-malate | activates by chelating and stabilizing Mn3+ | Lentinula edodes | |
| L-Tartrate | stimulates by chelating and stabilizing Mn3+ | Lentinula edodes | |
| malonate | stimulates by chelating and stabilizing Mn3+ | Lentinula edodes | |
| methylmalonate | stimulates by chelating and stabilizing Mn3+ | Lentinula edodes | |
| additional information | no Mn3+ chelators and activators: acetate, propionate, citrate, D-malate, ethylene glycol | Lentinula edodes | |
| additional information | succinate is no Mn3+ chelator and activator | Lentinula edodes | |
| oxalate | activates by chelating and stabilizing Mn3+ | Lentinula edodes | |
| oxaloacetate | stimulates by chelating and stabilizing Mn3+ | Lentinula edodes | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by 0.1 mM Cu2+, Co2+ or salicylic acid; not inhibited by salicylic acid | Lentinula edodes |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Lentinula edodes | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44600 | - |
x * 44600, SDS-PAGE | Lentinula edodes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lentinula edodes | - |
syn. Lentinus edodes, heterodikaryon strain 861 | - |
| Lentinula edodes | - |
white rot basidomycete, produces edible shiitake mushroom | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| MnP1: 19fold purification | Lentinula edodes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 660 | - |
o-toluidine as substrate for Mn3+-oxidation | Lentinula edodes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Mn2+ + H+ + H2O2 | - |
Lentinula edodes | Mn3+ + H2O | chelating organic acids facilitate the dissociation of Mn3+ from enzyme | ? | |
| Mn2+ + H+ + H2O2 | - |
Lentinula edodes | Mn3+ + H2O | Mn3+ oxidizes phenolic lignin model compounds | ? | |
| Mn2+ + H+ + H2O2 | - |
Lentinula edodes | Mn3+ + H2O | Mn3+ oxidizes vanillylacetone | ? | |
| Mn2+ + H+ + H2O2 | - |
Lentinula edodes | Mn3+ + H2O | chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential | ? | |
| additional information | enzyme is able to oxidatively depolymerize both dimeric lignin-model compounds and milled spruce-wood lignin | Lentinula edodes | ? | - |
? | |
| additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Lentinula edodes | ? | - |
? | |
| additional information | enzyme oxidizes veratryl alcohol and o-tolidine | Lentinula edodes | ? | - |
? | |
| additional information | in presence of H2O2 and Mn2+ the enzyme oxidizes lignin and lignin-model compounds | Lentinula edodes | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44600, SDS-PAGE | Lentinula edodes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Lentinula edodes |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 65 | progressive enhancement of activity from 20°C to 65°C | Lentinula edodes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
complete inactivation after boiling for 5 min | Lentinula edodes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 3.2 | Lentinula edodes |
| 3.5 | 5.5 | - |
Lentinula edodes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | heme-iron protein | Lentinula edodes | |
| heme | one iron protoporphyrin IX prosthetic group per enzyme molecule | Lentinula edodes | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
