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Literature summary for 1.11.1.12 extracted from

  • Yu, Y.; Song, J.; Guo, X.; Wang, S.; Yang, X.; Chen, L.; Wei, J.
    Characterization and structural analysis of human selenium-dependent glutathione peroxidase 4 mutant expressed in Escherichia coli (2014), Free Radic. Biol. Med., 71, 332-338 .
    View publication on PubMed
Search for more literature for 1.11.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of mutant C2S/C10S/C37S/C66S/C75S/C107S/C148S. The catalytic tetrad consists of residues Sec46, Gln81, Trp136, and Asn137 Homo sapiens

Protein Variants

Protein Variants Comment Organism
C2S/C10S/C37S/C66S/C75S/C107S/C148S substitution of all Cys residues by Ser. The mutant exhibits a Ping-Pong mechanism and structure similar to that of native GPx4, the activity is similar to wild-type Homo sapiens
additional information substitution of residues Cys2, Cys37, Cys75, Cys107, and Cys148 with Sec leads to loss of activity. The catalytic activity of GPx4 mutants increases as the number of noncatalytic Sec residues decreases Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P36969
-
-

Subunits

Subunits Comment Organism
? x * 20000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
GPx4
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 mutant C2S/C10S/C37S/C66S/C75S/C107S/C148S Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 mutant C2S/C10S/C37S/C66S/C75S/C107S/C148S Homo sapiens