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Literature summary for 1.11.1.12 extracted from
- Combining crystallography and molecular dynamics: The case of Schistosoma mansoni phospholipid glutathione peroxidase (2009), Proteins, 78, 259-270.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the codon optimized gene is cloned into the vector pGex4T-1 for expression in Escherichia coli BL21DE3 cells | Schistosoma mansoni |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the crystal structures of the Sec43Cys and Sec43Ser mutants are solved at resolutions of 1.0 A and 1.7 A, respectively | Schistosoma mansoni |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Sec43Cys | mutation of the selenium cysteine in the active site | Schistosoma mansoni |
| Sec43Ser | mutation of the selenium cysteine in the active site | Schistosoma mansoni |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| selenium | - |
Schistosoma mansoni |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19400 | - |
determined by gel filtration | Schistosoma mansoni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + a lipid hydroperoxide | Schistosoma mansoni | - |
glutathione disulfide + lipid + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schistosoma mansoni | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sec43Cys is purified on a GSH-Sepharose column, Sec43Ser is purified by ionic exchange chromatography, the GST-tags are cleaved with bovine thrombin | Schistosoma mansoni |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| integument | - |
Schistosoma mansoni | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + a lipid hydroperoxide | - |
Schistosoma mansoni | glutathione disulfide + lipid + H2O | - |
? | |
| glutathione + H2O2 | activity assay | Schistosoma mansoni | ? | - |
? | |
| glutathione + tert-butyl-hydroperoxide | activity assay | Schistosoma mansoni | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Schistosoma mansoni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| class 4 GPx | - |
Schistosoma mansoni |
| phospholipid glutathione peroxidase | - |
Schistosoma mansoni |
| seleno GPx | - |
Schistosoma mansoni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
activity assay | Schistosoma mansoni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
activity assay | Schistosoma mansoni |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | - |
Schistosoma mansoni | |
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Release 2023.1 (January 2023)
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