Cloned (Comment) | Organism |
---|---|
the entire encoding region for Oryza sativa PHGPx is expressed in Escherichia coli M15 | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00014 | - |
1-palmitoyl-2-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-L-3-phosphatidylcholine | cosubstrate: glutathione | Oryza sativa | |
0.0362 | - |
1-palmitoyl-2-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-L-3-phosphatidylcholine | cosubstrate: thioredoxin | Oryza sativa | |
0.799 | - |
H2O2 | cosubstrate: glutathione | Oryza sativa | |
4.344 | - |
tert-butyl hydroperoxide | cosubstrate: glutathione | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
x * 21000, recombinant enzyme containing a six-histidine tag at its N-terminus, SDS-PAGE | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-palmitoyl-2-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-L-3-phosphatidylcholine | - |
Oryza sativa | ? | - |
? | |
glutathione + H2O2 | - |
Oryza sativa | ? | - |
? | |
glutathione + tert-butyl hydroperoxide | - |
Oryza sativa | ? | - |
? | |
thioredoxin + 1-palmitoyl-2-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-L-3-phosphatidylcholine | - |
Oryza sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 21000, recombinant enzyme containing a six-histidine tag at its N-terminus, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
OsPHGPx | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
- |
Oryza sativa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | 10°C: 80% of maximal activity, 40°C: 60% of maximal activity | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Oryza sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | pH 8.0: about 40% of maximal activity, pH 11: about 80% of maximal activity | Oryza sativa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Oryza sativa | isoelectric focusing electrophoresis | - |
8.5 |