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Literature summary for 1.11.1.12 extracted from
- Purification and characterization of phospholipid hydroperoxide glutathione peroxidase from rat testis mitochondrial membranes (1994), Biochim. Biophys. Acta, 1208, 211-221.
General Stability
| General Stability | Organism |
|---|---|
| presence of thiol, e.g. 2-mercaptoethanol, during purification is necessary for stabilization | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Se | - |
Rattus norvegicus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + lipid hydroperoxide | Rattus norvegicus | - |
glutathione disulfide + lipid + H2O | - |
? | |
| additional information | Rattus norvegicus | controlled by gonadotropin | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from cytosol and mitochondria | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| testis | strongly linked to mitochondria of cells undergoing differentiation to spermatozoa, under gonadotropin control | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity of cytosolic and mitochondrial enzymes with several hydroperoxide substrates and reducing agents | Rattus norvegicus |
| 109900 | - |
purified enzyme from cytosol | Rattus norvegicus |
| 146300 | - |
purified enzyme from mitochondria | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + cumene hydroperoxide | - |
Rattus norvegicus | ? | - |
? | |
| glutathione + H2O2 | - |
Rattus norvegicus | ? | - |
? | |
| glutathione + L-alpha-phosphatidylcholine hydroperoxide | - |
Rattus norvegicus | ? | - |
? | |
| glutathione + LDL hydroperoxides | - |
Rattus norvegicus | ? | - |
? | |
| glutathione + lipid hydroperoxide | - |
Rattus norvegicus | glutathione disulfide + lipid + H2O | - |
? | |
| glutathione + lipid hydroperoxide | dithiothreitol and dithioerythritol are 3fold more active than glutathione, while 2-mercaptoethanol and L-cysteine show 50% and 20% of the activity with glutathione as reductants, respectively | Rattus norvegicus | glutathione disulfide + lipid + H2O | - |
? | |
| glutathione + tert-butyl hydroperoxide | - |
Rattus norvegicus | ? | - |
? | |
| additional information | controlled by gonadotropin | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 22000, SDS-PAGE | Rattus norvegicus |
| More | cytosolic enzyme shows also a minor component in SDS-PAGE at higher molecular weight, which is not further characterized | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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