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Literature summary for 1.11.1.12 extracted from
- Bacterial and nonbacterial expression of wild-type and mutant human phospholipid hydroperoxide glutathione peroxidase and purification of the mutant enzyme in the milligram scale (2000), Protein Expr. Purif., 19, 403-410.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type enzyme and SeC46C mutant in baculovirus/insect cell system and expression of mutant SeC46C as His-tagged fusion protein in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| SeC46C | expression of mutant in Escherichia coli as His-tagged fusion protein and in baculovirus/insect cell system, site directed mutagenesis to avoid recognition problems of selenocysteine encoded by TGA stop codon in the expression systems, mutant has reduced specific acitvity | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Se | enzyme contains a selenocysteine residue at the active site | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + lipid hydroperoxide | Homo sapiens | - |
glutathione disulfide + lipid + H2O | - |
? | |
| additional information | Homo sapiens | phospholipid hydroperoxide glutathione peroxidase and 15-lipoxygenase are counterparts in the metabolism of hydroperoxy lipids | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| large-scale, wild-type and mutant SeC46C from baculovirus/insect cell expression system and mutant SeC46C from expression in Escherichia coli as His-tagged fusion protein | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
mutant compared to wild-type enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + dilinoleoyl phosphatidylcholine hydroperoxide | - |
Homo sapiens | ? | - |
? | |
| glutathione + lipid hydroperoxide | - |
Homo sapiens | glutathione disulfide + lipid + H2O | - |
? | |
| additional information | phospholipid hydroperoxide glutathione peroxidase and 15-lipoxygenase are counterparts in the metabolism of hydroperoxy lipids | Homo sapiens | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | mutant compared to wild-type enzyme | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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