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Literature summary for 1.11.1.11 extracted from

  • Kuo, E.Y.; Cai, M.S.; Lee, T.M.
    Ascorbate peroxidase 4 plays a role in the tolerance of Chlamydomonas reinhardtii to photo-oxidative stress (2020), Sci. Rep., 10, 13287 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.11

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Chlamydomonas reinhardtii 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ in the heme group Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-ascorbate + H2O2 + 2 H+ Chlamydomonas reinhardtii
-
 L-ascorbate + L-dehydroascorbate + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-ascorbate + H2O2 + 2 H+
-
 Chlamydomonas reinhardtii L-ascorbate + L-dehydroascorbate + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
APX4
-
 Chlamydomonas reinhardtii
ascorbate peroxidase 4
-
 Chlamydomonas reinhardtii
CrAPX4
-
 Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
heme
-
 Chlamydomonas reinhardtii

Expression

Organism Comment Expression
Chlamydomonas reinhardtii CrAPX4 is downregulated using amiRNA technology to examine the role of APX for high-light (HL) acclimation. The CrAPX4 knockdown amiRNA lines show low APX activity and CrAPX4 transcript level without a change in CrAPX1 and CrAPX2 transcript levels, and monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR) activities and transcript levels. Upon exposure to high-light (HL), CrAPX4 knockdown amiRNA lines show a modification in the expression of genes encoding the enzymes in the ascorbate-glutathione cycle, including an increase in transcript level of CrVTC2, a key enzyme for ascorbate (AsA) biosynthesis but a decrease in MDAR and DHAR transcription and activity after 1 h, followed by increases in reactive oxygen species production and lipid peroxidation after 6 h, and exhibit cell death after 9 h. Besides, AsA content and AsA/DHA (dehydroascorbate) ratio decrease in CrAPX4 knockdown amiRNA lines after prolonged HL treatment additional information

General Information

General Information Comment Organism
malfunction CrAPX4 knockdown amiRNA lines show low APX activity and CrAPX4 transcript level without a change in CrAPX1 and CrAPX2 transcript levels, and monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR) activities and transcript levels. Upon exposure to high-light (HL), CrAPX4 knockdown amiRNA lines show a modification in the expression of genes encoding the enzymes in the ascorbate-glutathione cycle, including an increase in transcript level of CrVTC2, a key enzyme for ascorbate (AsA) biosynthesis but a decrease in MDAR and DHAR transcription and activity after 1 h, followed by increases in reactive oxygen species production and lipid peroxidation after 6 h, and exhibit cell death after 9 h. Besides, AsA content and AsA/DHA (dehydroascorbate) ratio decrease in CrAPX4 knockdown amiRNA lines after prolonged HL treatment Chlamydomonas reinhardtii
physiological function isozyme CrAPX4 induction together with its association with the modulation of MDAR and DHAR expression for AsA regeneration is critical for Chlamydomonas to cope with photooxidative stress. APX is a central enzyme for ROS scavenging in plants can be induced under abiotic and biotic stresses Chlamydomonas reinhardtii