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Literature summary for 1.11.1.11 extracted from

  • Hugo, M.; Martinez, A.; Trujillo, M.; Estrada, D.; Mastrogiovanni, M.; Linares, E.; Augusto, O.; Issoglio, F.; Zeida, A.; Estrin, D.A.; Heijnen, H.F.; Piacenza, L.; Radi, R.
    Kinetics, subcellular localization, and contribution to parasite virulence of a Trypanosoma cruzi hybrid type A heme peroxidase (TcAPx-CcP) (2017), Proc. Natl. Acad. Sci. USA, 114, E1326-E1335 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Trypanosoma cruzi

Protein Variants

Protein Variants Comment Organism
W233F complete loss of cytochrome c-dependent activity Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 cytochrome c pH 7.4, 25°C Trypanosoma cruzi
0.19
-
 L-ascorbate pH 7.4, 25°C Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion enzyme is closely associated with mitochondrial membranes Trypanosoma cruzi 5739
-
plasma membrane enzyme is associated with plasma membrane Trypanosoma cruzi 5886
-

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q8I1N3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-ascorbate + H2O2 + 2 H+
-
 Trypanosoma cruzi 2 monodehydroascorbate + 2 H2O
-
 ?
cytochrome c + H2O2
-
 Trypanosoma cruzi ? + H2O
-
 ?
additional information reaction includes formation of a compound I-like product, characteristic of the generation of a tryptophanyl radical-cation at residue W233. In addition, formation of a C222-derived radical is observed. electron transfer between Trp233 and Cys222 is possible and likely to participate in the catalytic cycle Trypanosoma cruzi ?
-
 ?

Synonyms

Synonyms Comment Organism
APX
-
 Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
heme
-
 Trypanosoma cruzi

General Information

General Information Comment Organism
physiological function overexpressing strains are significantly more infective to macrophages and cardiomyocytes, as well as in the mouse model of Chagas disease than wild-type Trypanosoma cruzi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
35
-
 L-ascorbate pH 7.4, 25°C Trypanosoma cruzi
210
-
 cytochrome c pH 7.4, 25°C Trypanosoma cruzi