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Literature summary for 1.11.1.11 extracted from

  • Zhang, M.; Gong, M.; Yang, Y.; Li, X.; Wang, H.; Zou, Z.
    Improvement on the thermal stability and activity of plant cytosolic ascorbate peroxidase 1 by tailing hyper-acidic fusion partners (2015), Biotechnol. Lett., 37, 891-898 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Arabidopsis thaliana
-
Jatropha curcas

Protein Variants

Protein Variants Comment Organism
additional information expression with hyperacidic fusion partners such as C-end tail of human alpha-synuclein, C-end tails of Arabidopsis tubulins, TUA2 and TUB3, Escherichia coli msyB and C-end tail of Escherichia coli yjgD efficiently improves the thermostability and prevents thermal inactivation of APX1 with an elevated heat tolerance of at least 2°C Arabidopsis thaliana
additional information expression with hyperacidic fusion partners such as C-end tail of human alpha-synuclein, C-end tails of Arabidopsis tubulins, TUA2 and TUB3, Escherichia coli msyB and C-end tail of Escherichia coli yjgD efficiently improves the thermostability and prevents thermal inactivation of APX1 with an elevated heat tolerance of at least 2°C Jatropha curcas

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Arabidopsis thaliana 5829
-
cytosol
-
Jatropha curcas 5829
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q05431
-
-
Jatropha curcas D3GC00
-
-

Synonyms

Synonyms Comment Organism
APX1
-
Arabidopsis thaliana
APX1
-
Jatropha curcas

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37
-
wild-type, decrease in activity above Arabidopsis thaliana
42
-
fusion protein with C-end tail of human alpha-synuclein, 50% residual activity Arabidopsis thaliana
42.3
-
wild-type, less than 10% residual activity Arabidopsis thaliana
47.7
-
wild-type, almost entire loss of activity Jatropha curcas
50.5
-
fusion forms with C-terminal hyperacidic tails, no notable loss of activity below Jatropha curcas
64.5
-
fusion protein with C-end tail of Arabidopsis tubulin TUA2, 50% residual activity Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42.3
-
wild-type, nearly 30 % of the solubility remains Arabidopsis thaliana
47.7
-
wild-type, loss of solubility Jatropha curcas