Literature summary for 1.11.1.11 extracted from

Hwang, J.; Espenshade, P.J.
Proximity-dependent biotin labelling in yeast using the engineered ascorbate peroxidase APEX2 (2016), Biochem. J., 473, 2463-2469 .

Search for more literature for 1.11.1.11

Application

Application	Comment	Organism
analysis	APEX2, i.e. engineered mutant K14D/W41F/E112K/A134P of APX, fused to a protein of interest covalently tags nearby proteins with biotin-phenol when H2O2 is added. High osmolarity and disruption of cell wall integrity permits live-cell biotin labeling in Schizosaccharomyces pombe and Saccharomyces cerevisiae, respectively. APEX2 permits targeted and proximity-dependent labeling of proteins	Glycine max

Cloned(Commentary)

Cloned (Comment)	Organism
-	Glycine max

Protein Variants

Protein Variants	Comment	Organism
K14D/W41F/E112K/A134P	APEX2, i.e. engineered mutant of APX. APEX2 fused to a protein of interest covalently tags nearby proteins with biotin-phenol when H2O2 is added. High osmolarity and disruption of cell wall integrity permits live-cell biotin labeling in Schizosaccharomyces pombe and Saccharomyces cerevisiae, respectively. APEX2 permits targeted and proximity-dependent labeling of proteins	Glycine max

Organism

Organism	UniProt	Comment	Textmining
Glycine max	Q76LA6	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Glycine max	-

Subunits

Subunits	Comment	Organism
?	x * 28000, SDS-PAGE, recombinant protein	Glycine max

Synonyms

Synonyms	Comment	Organism
APEX2	-	Glycine max

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Release 2023.1 (January 2023)