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Literature summary for 1.11.1.11 extracted from
- Purification and kinetic characterization of the liverwort Pallavicinia lyelli (Hook.) S. Gray. cytosolic ascorbate peroxidase (2010), Plant Physiol. Biochem., 48, 758-763.
General Stability
| General Stability | Organism |
|---|---|
| when the enzyme is diluted with the ascorbate-deleted medium, the half inactivation time is approximately 15 min | Pallavicinia lyellii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | 96% inhibition at 0.5 mM | Pallavicinia lyellii |  |
| azide | 46% inhibition at 0.5 mM | Pallavicinia lyellii | |
| beta-mercaptoethanol | 29% inhibition at 3 mM | Pallavicinia lyellii | |
| cyanide | - |
Pallavicinia lyellii | |
| diethylenetriamine pentaacetic acid | 9% inhibition at 5 mM | Pallavicinia lyellii | |
| dithioerythritol | 37% inhibition at 3 mM | Pallavicinia lyellii | |
| dithiothreitol | 40% inhibition at 3 mM | Pallavicinia lyellii | |
| EDTA | 97% inhibition at 3 mM | Pallavicinia lyellii | |
| Hydroxyurea | - |
Pallavicinia lyellii | |
| iodoacetamide | 19% inhibition at 3 mM | Pallavicinia lyellii | |
| L-cysteine | 28% inhibition at 3 mM | Pallavicinia lyellii | |
| p-chloromercuribenzoate | 27% inhibition at 3 mM | Pallavicinia lyellii | |
| reduced glutathione | 25% inhibition at 3 mM | Pallavicinia lyellii | |
| salicylic acid | 98% inhibition at 0.5 mM | Pallavicinia lyellii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0287 | - |
H2O2 | in 50 mM potassium phosphate (pH 7.0), at 22°C | Pallavicinia lyellii | |
| 0.123 | - |
L-ascorbate | in 50 mM potassium phosphate (pH 7.0), at 22°C | Pallavicinia lyellii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | approximately 46% of the APX activity is associated with intact chloroplasts | Pallavicinia lyellii | 9507 | - |
| cytosol | 54% of the total APX activity is presumed to be cytosolic APX | Pallavicinia lyellii | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
gel filtration | Pallavicinia lyellii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pallavicinia lyellii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, butyl-Toyopearl column chromatography, DEAE-cellulofine column chromatography, and Sephadex G-75 gel filtration | Pallavicinia lyellii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thallus | - |
Pallavicinia lyellii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.6 | - |
crude extract, in 50 mM potassium phosphate (pH 7.0), at 22°C | Pallavicinia lyellii |
| 456 | - |
after 285fold purification, in 50 mM potassium phosphate (pH 7.0), at 22°C | Pallavicinia lyellii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | cytosolic ascorbate peroxidase shows 3% activity compared to L-ascorbate, in the presence of 0.1 mM H2O2 and 3.6% activity compared to L-ascorbate in the presence of 0.5 mM H2O2 | Pallavicinia lyellii | ? | - |
? | |
| guaiacol + H2O2 | cytosolic ascorbate peroxidase shows 12% activity compared to L-ascorbate, in the presence of 0.1 mM H2O2 and 24.7% activity compared to L-ascorbate in the presence of 0.5 mM H2O2 | Pallavicinia lyellii | ? | - |
? | |
| L-ascorbate + H2O2 | cytosolic ascorbate peroxidase shows 100% activity in the presence of 0.1 mM and 0.5 mM H2O2 | Pallavicinia lyellii | dehydroascorbate + H2O | - |
? | |
| additional information | cytosolic and chloroplastic ascorbate peroxidase shows no activity with tert-butyl hydroperoxide and cumene hydroperoxide, pyrocatechol, hydroxyurea, GSH, cytochrome c, NADH, and NADPH. Chlorplastic ascorbate peroxidase displays no activity with pyrogallol, guaiacol, pyrocatechol, and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) | Pallavicinia lyellii | ? | - |
? | |
| pyrogallol + H2O2 | cytosolic ascorbate peroxidase shows 29% activity compared to L-ascorbate, in the presence of 0.1 mM H2O2 and 208% activity compared to L-ascorbate in the presence of 0.5 mM H2O2 | Pallavicinia lyellii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28000, SDS-PAGE | Pallavicinia lyellii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| APX | - |
Pallavicinia lyellii |
| ascorbate peroxidase | - |
Pallavicinia lyellii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pallavicinia lyellii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
the enzyme is relatively stable at 60°C, with 54% loss of activity | Pallavicinia lyellii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 760 | - |
L-ascorbate | in 50 mM potassium phosphate (pH 7.0), at 22°C | Pallavicinia lyellii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
cytosolic APX | Pallavicinia lyellii |
| 8 | - |
chloroplast APX | Pallavicinia lyellii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | the activity of APX decreases rapidly at pH above 8.0 | Pallavicinia lyellii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | the enzyme is not stable below pH 5.0 and above 8.0 for 24 h at 4°C, 68% of the activity is retained between pH 5.0 and 8.0 for 24 h at 4°C | Pallavicinia lyellii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Pallavicinia lyellii | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6600 | - |
L-ascorbate | in 50 mM potassium phosphate (pH 7.0), at 22°C | Pallavicinia lyellii | |
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