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Literature summary for 1.11.1.11 extracted from

  • Reddy, R.A.; Kumar, B.; Reddy, P.S.; Mishra, R.N.; Mahanty, S.; Kaul, T.; Nair, S.; Sopory, S.K.; Reddy, M.K.
    Molecular cloning and characterization of genes encoding Pennisetum glaucum ascorbate peroxidase and heat-shock factor: interlinking oxidative and heat-stress responses (2009), J. Plant Physiol., 166, 1646-1659.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET-28a+ for the expression in Escherichia coli BL21DE3 cells, the 5'-flanking region of the PgAPX1 gene is cloned into the Topo-TA vector Cenchrus americanus

General Stability

General Stability Organism
the enzyme is very unstable in the absence of ascorbate and looses its activity within min particularly in the case of chloroplastic and mitochondrial isoforms Cenchrus americanus

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine
-
Cenchrus americanus
salicylic acid
-
Cenchrus americanus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
ascorbate recombinant PgAPX Cenchrus americanus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Cenchrus americanus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
determined by SDS-PAGE Cenchrus americanus
27500
-
apparent molecular weight Cenchrus americanus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ascorbate + H2O2 Cenchrus americanus
-
dehydroascorbate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Cenchrus americanus A4ZYP9
-
-

Purification (Commentary)

Purification (Comment) Organism
purified near homogeneity from clarified Escherichia coli lysate by passing through Ni-NTA agarose beads Cenchrus americanus

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Cenchrus americanus
-
seedling
-
Cenchrus americanus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ascorbate + H2O2
-
Cenchrus americanus dehydroascorbate + H2O
-
?

Synonyms

Synonyms Comment Organism
ascorbate peroxidase
-
Cenchrus americanus
cytoplasmic ascorbate peroxidase 1
-
Cenchrus americanus
PgAPX1
-
Cenchrus americanus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
in gel activity assay at room temperature Cenchrus americanus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
in gel activity assay Cenchrus americanus
6.6
-
activity 75 units mg-1 protein min-1 Cenchrus americanus

pH Range

pH Minimum pH Maximum Comment Organism
5 7.8 activity 35 units mg-1 protein min-1 at pH 5.0 and pH 7.8 Cenchrus americanus

Cofactor

Cofactor Comment Organism Structure
heme
-
Cenchrus americanus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0308
-
hydroxylamine
-
Cenchrus americanus
0.1905
-
salicylic acid
-
Cenchrus americanus

pI Value

Organism Comment pI Value Maximum pI Value
Cenchrus americanus estimated isoelectric point
-
5.62

Expression

Organism Comment Expression
Cenchrus americanus expression of PgAPX1 significantly increases under heat-stress conditions, transcript analysis suggests that the heat-shock element, HSE, motifs may be responsible for stress-specific expression of PgAPX1 by interacting with PgHSF up