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Literature summary for 1.11.1.11 extracted from

  • Meharenna, Y.T.; Oertel, P.; Bhaskar, B.; Poulos, T.L.
    Engineering ascorbate peroxidase activity into cytochrome c peroxidase (2008), Biochemistry, 47, 10324-10332.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the vector pT-7 is used, cytochrome c peroxidase, CCP, is converted into an ascorbate peroxidase, APX, by engineering the ascorbate-binding loop and critical arginine into CCP to give the CCP2APX mutant Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structures of the CCP2APX and CCP2APX/F191 mutants are solved Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
CCP2APX residues 30-42, LREDDEYDNYIGY, of wild-type CCP are replaced with residues 27-32, IAEKKC, of APX in order to introduce the ascorbate-binding loop, a N184R point mutation is added Saccharomyces cerevisiae
CCP2APX/F191 in order to enable CCP2APX to form a porphyrin pi-cation radical during catalysis, Trp191 is converted to Phe Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ascorbate + H2O2 Saccharomyces cerevisiae
-
dehydroascorbate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Escherichia coli mutants are purified by chromatography on a Sephadex G-75 column and a DEAE-Sephacel anione-exchange column Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ascorbate + H2O2
-
Saccharomyces cerevisiae dehydroascorbate + H2O
-
?

Synonyms

Synonyms Comment Organism
APX
-
Saccharomyces cerevisiae
ascorbate peroxidase
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.5
-
activity assay Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
ascorbate CCP2APX Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
heme
-
Saccharomyces cerevisiae