Cloned (Comment) | Organism |
---|---|
the vector pT-7 is used, cytochrome c peroxidase, CCP, is converted into an ascorbate peroxidase, APX, by engineering the ascorbate-binding loop and critical arginine into CCP to give the CCP2APX mutant | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
the crystal structures of the CCP2APX and CCP2APX/F191 mutants are solved | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
CCP2APX | residues 30-42, LREDDEYDNYIGY, of wild-type CCP are replaced with residues 27-32, IAEKKC, of APX in order to introduce the ascorbate-binding loop, a N184R point mutation is added | Saccharomyces cerevisiae |
CCP2APX/F191 | in order to enable CCP2APX to form a porphyrin pi-cation radical during catalysis, Trp191 is converted to Phe | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ascorbate + H2O2 | Saccharomyces cerevisiae | - |
dehydroascorbate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant Escherichia coli mutants are purified by chromatography on a Sephadex G-75 column and a DEAE-Sephacel anione-exchange column | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ascorbate + H2O2 | - |
Saccharomyces cerevisiae | dehydroascorbate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APX | - |
Saccharomyces cerevisiae |
ascorbate peroxidase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
ascorbate | CCP2APX | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Saccharomyces cerevisiae |