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Literature summary for 1.11.1.11 extracted from
- Purification, properties, and distribution of ascorbate peroxidase in legume root nodules (1987), Plant Physiol., 83, 789-794.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| C2H2 | potent inhibitor | Glycine max |  |
| CO | potent inhibitor | Glycine max | |
| KCN | potent inhibitor | Glycine max | |
| NaN3 | potent inhibitor | Glycine max | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Pisum sativum | |
| additional information | - |
additional information | the reaction does not follow Michaelis-Menten kinetics, Hill plots | Glycine max |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Vicia faba | 5829 | - |
| cytosol | - |
Vicia sativa | 5829 | - |
| cytosol | - |
Pisum sativum | 5829 | - |
| cytosol | - |
Glycine max | 5829 | - |
| cytosol | - |
Arachis hypogaea | 5829 | - |
| cytosol | - |
Medicago sativa | 5829 | - |
| cytosol | - |
Lupinus albus | 5829 | - |
| cytosol | - |
Vigna unguiculata | 5829 | - |
| cytosol | - |
Trifolium subterraneum | 5829 | - |
| cytosol | - |
Alnus rubra | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | hemoprotein | Vicia faba | |
| Fe | hemoprotein | Vicia sativa | |
| Fe | hemoprotein | Pisum sativum | |
| Fe | hemoprotein | Glycine max | |
| Fe | hemoprotein | Arachis hypogaea | |
| Fe | hemoprotein | Medicago sativa | |
| Fe | hemoprotein | Lupinus albus | |
| Fe | hemoprotein | Vigna unguiculata | |
| Fe | hemoprotein | Trifolium subterraneum | |
| Fe | hemoprotein | Alnus rubra | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
1 * 30000, SDS-PAGE, discrepancy to value from gel filtration probably due to enzyme conformation | Glycine max |
| 47000 | - |
- |
Vicia faba |
| 47000 | - |
- |
Vicia sativa |
| 47000 | - |
- |
Pisum sativum |
| 47000 | - |
- |
Arachis hypogaea |
| 47000 | - |
- |
Medicago sativa |
| 47000 | - |
- |
Lupinus albus |
| 47000 | - |
- |
Vigna unguiculata |
| 47000 | - |
- |
Trifolium subterraneum |
| 47000 | - |
- |
Alnus rubra |
| 47000 | - |
gel filtration | Glycine max |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ascorbate + H2O2 | Pisum sativum | - |
dehydroascorbate + 2 H2O | - |
? | |
| L-ascorbate + H2O2 | Glycine max | the enzyme is responsible for most H2O2 removal outside of peroxisomes in root nodules | dehydroascorbate + 2 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alnus rubra | - |
- |
- |
| Arachis hypogaea | - |
- |
- |
| Glycine max | - |
soybean | - |
| Lupinus albus | - |
low activity | - |
| Medicago sativa | - |
alfalfa | - |
| Pisum sativum | - |
pea | - |
| Pisum sativum | - |
cv Alaska and cv Austrian winter | - |
| Trifolium subterraneum | - |
- |
- |
| Vicia faba | - |
- |
- |
| Vicia sativa | - |
- |
- |
| Vigna unguiculata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using ammonium sulfate precipitation, dialysis and column chromatography on DEAE, Sephacryl und hydroxyapatite | Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | nodules | Vicia faba | - |
| root | nodules | Vicia sativa | - |
| root | nodules | Pisum sativum | - |
| root | nodules | Glycine max | - |
| root | nodules | Arachis hypogaea | - |
| root | nodules | Medicago sativa | - |
| root | nodules | Lupinus albus | - |
| root | nodules | Vigna unguiculata | - |
| root | nodules | Trifolium subterraneum | - |
| root | nodules | Alnus rubra | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.01 | - |
crude extract of root nodules, the activity is rapidly lost after extraction | Lupinus albus |
| 0.092 | - |
crude extract of root nodules | Trifolium subterraneum |
| 0.096 | - |
crude extract of root nodules, cv Alaska | Pisum sativum |
| 0.11 | - |
crude extract of root nodules | Medicago sativa |
| 0.161 | - |
crude extract of root nodules, cv Austrian winter | Pisum sativum |
| 0.213 | - |
crude extract of root nodules | Vicia sativa |
| 0.241 | - |
crude extract of root nodules | Vicia faba |
| 0.258 | - |
crude extract of root nodules | Vigna unguiculata |
| 0.284 | - |
crude extract of root nodules | Glycine max |
| 0.366 | - |
crude extract of root nodules | Arachis hypogaea |
| 4.32 | - |
crude extract of root nodules, activity is only detected when soluble polyvinylpolypyrrolidone is included in the buffer and O2 is excluded by through degassing of buffers and performing all extraction steps under a vigorous stream of N2 gas | Alnus rubra |
| 34.2 | - |
purified enzyme | Glycine max |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C or -80°C, crude extracts of root nodules, 50 mM potassium phosphate buffer, pH 7.0, several months | Glycine max |
| -80°C, intact soybean nodules, stable, 3 years | Glycine max |
| 4°C, crude extracts of root nodules, in air, 25% loss of activity, 15 days | Glycine max |
| 4°C, crude extracts of root nodules, in air, stable, 6 days | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-di-[3-ethylbenzothiazoline-(6)-sulfonic acid] + H2O2 | - |
Vicia faba | ? | - |
? | |
| 2,2'-azino-di-[3-ethylbenzothiazoline-(6)-sulfonic acid] + H2O2 | - |
Vicia sativa | ? | - |
? | |
| 2,2'-azino-di-[3-ethylbenzothiazoline-(6)-sulfonic acid] + H2O2 | - |
Pisum sativum | ? | - |
? | |
| 2,2'-azino-di-[3-ethylbenzothiazoline-(6)-sulfonic acid] + H2O2 | - |
Glycine max | ? | - |
? | |
| 2,2'-azino-di-[3-ethylbenzothiazoline-(6)-sulfonic acid] + H2O2 | - |
Arachis hypogaea | ? | - |
? | |
| 2,2'-azino-di-[3-ethylbenzothiazoline-(6)-sulfonic acid] + H2O2 | - |
Medicago sativa | ? | - |
? | |
| 2,2'-azino-di-[3-ethylbenzothiazoline-(6)-sulfonic acid] + H2O2 | - |
Lupinus albus | ? | - |
? | |
| 2,2'-azino-di-[3-ethylbenzothiazoline-(6)-sulfonic acid] + H2O2 | - |
Vigna unguiculata | ? | - |
? | |
| 2,2'-azino-di-[3-ethylbenzothiazoline-(6)-sulfonic acid] + H2O2 | - |
Trifolium subterraneum | ? | - |
? | |
| 2,2'-azino-di-[3-ethylbenzothiazoline-(6)-sulfonic acid] + H2O2 | - |
Alnus rubra | ? | - |
? | |
| glutathione + H2O2 | no activity | Glycine max | ? + H2O | - |
? | |
| guaiacol + H2O2 | - |
Pisum sativum | ? | - |
? | |
| guaiacol + H2O2 | the reaction rate is approximately equal to the rate with L-ascorbate | Glycine max | ? | - |
? | |
| L-ascorbate + H2O2 | - |
Vicia faba | dehydroascorbate + H2O | - |
? | |
| L-ascorbate + H2O2 | - |
Vicia sativa | dehydroascorbate + H2O | - |
? | |
| L-ascorbate + H2O2 | - |
Pisum sativum | dehydroascorbate + H2O | - |
? | |
| L-ascorbate + H2O2 | - |
Glycine max | dehydroascorbate + H2O | - |
? | |
| L-ascorbate + H2O2 | - |
Arachis hypogaea | dehydroascorbate + H2O | - |
? | |
| L-ascorbate + H2O2 | - |
Medicago sativa | dehydroascorbate + H2O | - |
? | |
| L-ascorbate + H2O2 | - |
Lupinus albus | dehydroascorbate + H2O | - |
? | |
| L-ascorbate + H2O2 | - |
Vigna unguiculata | dehydroascorbate + H2O | - |
? | |
| L-ascorbate + H2O2 | - |
Trifolium subterraneum | dehydroascorbate + H2O | - |
? | |
| L-ascorbate + H2O2 | - |
Alnus rubra | dehydroascorbate + H2O | - |
? | |
| L-ascorbate + H2O2 | - |
Pisum sativum | dehydroascorbate + 2 H2O | - |
? | |
| L-ascorbate + H2O2 | the enzyme is responsible for most H2O2 removal outside of peroxisomes in root nodules | Glycine max | dehydroascorbate + 2 H2O | - |
? | |
| additional information | - |
Pisum sativum | ? | - |
? | |
| additional information | no activity with: NAD(P)H, reduced glutathione or urate | Glycine max | ? | - |
? | |
| NADPH + H2O2 | no activity | Glycine max | ? + H2O | - |
? | |
| o-dianisidine + H2O2 | reaction rate approximately equal to the rate with L-ascorbate | Glycine max | ? | - |
? | |
| pyrogallol + H2O2 | - |
Pisum sativum | 3-hydroxybenzo-1,2-quinone + H2O | - |
? | |
| pyrogallol + H2O2 | the reaction rate is 38-fold higher than the rate with L-ascorbate | Glycine max | 3-hydroxybenzo-1,2-quinone + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE, discrepancy to value from gel filtration probably due to enzyme conformation | Glycine max |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
half-life 10 h | Vicia faba |
| 4 | - |
half-life 10 h | Vicia sativa |
| 4 | - |
half-life 10 h | Pisum sativum |
| 4 | - |
half-life 10 h | Glycine max |
| 4 | - |
half-life 10 h | Arachis hypogaea |
| 4 | - |
half-life 10 h | Medicago sativa |
| 4 | - |
half-life 10 h | Lupinus albus |
| 4 | - |
half-life 10 h | Vigna unguiculata |
| 4 | - |
half-life 10 h | Trifolium subterraneum |
| 4 | - |
half-life 10 h | Alnus rubra |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Vicia faba | |
| heme | - |
Vicia sativa | |
| heme | - |
Pisum sativum | |
| heme | - |
Glycine max | |
| heme | - |
Arachis hypogaea | |
| heme | - |
Medicago sativa | |
| heme | - |
Lupinus albus | |
| heme | - |
Vigna unguiculata | |
| heme | - |
Trifolium subterraneum | |
| heme | - |
Alnus rubra | |
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