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BRENDA support

Literature summary for 1.11.1.10 extracted from

  • Garcia-Embid, S.; Di Renzo, F.; De Matteis, L.; Spreti, N.; M. de la Fuente, J.
    Magnetic separation and high reusability of chloroperoxidase entrapped in multi polysaccharide micro-supports (2018), Appl. Catal. B, 560, 94-102 .
No PubMed abstract available
Search for more literature for 1.11.1.10

Protein Variants

Protein Variants Comment Organism
additional information chloroperoxidase is successfully entrapped in multi polysaccharide magnetic supports, which are developed based on a multi polysaccharide shell. Leakage of the enzyme is observed with single-shell polysaccharide coating. High CPO reusability is measured with a polymeric double-shell magnetic microsupport. The magnetic cores contain iron oxide nanoparticles, the polymer diminishes the interaction between the magnetic cores themselves, can improve the colloidal stability of the support, and prevent any interaction with the environment that would affect both support properties and enzyme stability. Different magnetic micro-supports, based on polydopamine-coated iron oxide nanoparticles with a multi polysaccharide shell, are developed and evaluated. Method overview. CPO is successfully immobilized with an efficiency of entrapment between 92% and 100% in the case of supports with chitosan in the interior or outer shell respectively. A very good chemical stability of the support under reaction conditions is observed in the case of an interior shell of alginate and an outer coating of chitosan, together with an excellent reusability of the immobilized enzyme, that is recycled to catalyze up to 25 consecutive reaction cycles. Chlorination of monochlorodimedone to dichlorodimedone is used as model reaction for chloroperoxidase catalytic assay Leptoxyphium fumago

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RH + chloride + H2O2 Leptoxyphium fumago
-
 RCl + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Leptoxyphium fumago P04963 Caldariomyces fumago
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chlorodimedone + chloride + H2O2 model substrate monochlorodimedone Leptoxyphium fumago 1,1-dimethyl-4,4-dichloro-3,5-cyclohexanedione + 2 H2O
-
 ?
RH + chloride + H2O2
-
 Leptoxyphium fumago RCl + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
chloroperoxidase
-
 Leptoxyphium fumago
CPO
-
 Leptoxyphium fumago

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Leptoxyphium fumago

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.9
-
 assay at Leptoxyphium fumago