Crystallization (Comment) | Organism |
---|---|
purified NQO2 in complex with primaquine and chloroquine, hanging drop vapor diffusion method, against reservoirs containing 0.1 M HEPES, pH 7.5, and 1.3-2.0 M (NH4)2SO4, crystals of NQO2-CQ are soaked in 0.001 ml of reducing-soak solution consisting of 0.1 M HEPES, pH 7.5, 2.0 M (NH4)2SO4, 10 mM 1-(3-sulfonatopropyl)-3-carbamoyl-1,4-dihydropyrimidine and 1 mM chloroquine, X-ray diffraction structure determination and analysis at 1.2-1.4 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Chloroquine | binds preferentially to reduced NQO2, binding mode, closure of a flexible loop (Phe126-Leu136) over the active site, overview | Homo sapiens | |
primaquine | binding mode, overview | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P16083 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
NQO2 | - |
Homo sapiens |
quinone reductase 2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavin redox switch, structural changes, overview | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | quinone reductase 2 is an FAD-linked enzyme and the only known human target of two antimalarial drugs, primaquine and chloroquine, a functional role for NQO2 as a flavin redox switch | Homo sapiens |