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Literature summary for 1.10.3.4 extracted from

  • Jia, H.; Gao, Z.; Ma, Y.; Zhong, C.; Xie, Y.; Zhou, H.; Wei, P.
    Optimization of phenoxazinone synthase production by response surface methodology and its application in Congo red decolourization (2013), Electron. J. Biotechnol., 16, 2.
No PubMed abstract available
Search for more literature for 1.10.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3). Optimization of phenoxazinone synthase production by response surface methodology via Box-Behnken design, overview Streptomyces antibioticus

Organism

Organism UniProt Comment Textmining
Streptomyces antibioticus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme from crude cell extract exhibits Congo red decolourization activity at a rate of 27% without mediator. Several mediators improve PHS-catalyzed Congo red decolourization, with the highest rate of 73.89% obtained with 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid as mediator under optimized conditions of 4000 U/l PHS activity, 0.01 mM mediator, 0.1 mM Congo red, and 8 h reaction time. 2,2'-Azinobis-3-ethylbenzothiazoline-6-sulfonic acid is also a substrate for the enzyme Streptomyces antibioticus ?
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Synonyms

Synonyms Comment Organism
phenoxazinone synthase
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 Streptomyces antibioticus
PHS
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 Streptomyces antibioticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
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 assay at Streptomyces antibioticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Streptomyces antibioticus