Literature summary for 1.10.3.3 extracted from

Nicolai, E.; Di Venere, A.; Rosato, N.; Rossi, A.; Finazzi Agro, A.; Mei, G.
Physico-chemical properties of molten dimer ascorbate oxidase (2006), FEBS J., 273, 5194-5204.

Search for more literature for 1.10.3.3

General Stability

General Stability	Organism
partially unfolding of the enzyme by 1.4 M guanidinium hydrochloride or 2.8 M urea	Cucurbita pepo

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	essential for activity, a tri-nuclear copper center structure, a partial loss of tertiary structure has strong effects on copper	Cucurbita pepo

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 L-ascorbic acid + O2	Cucurbita pepo	-	2 L-dehydroascorbic acid + 2 H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Cucurbita pepo	P37064	green zucchini	-

Reaction

Reaction	Comment	Organism	Reaction ID
4 L-ascorbate + O2 = 4 monodehydroascorbate + 2 H2O	the transition from native dimer to the dimeric intermediate is characterized by the release of copper ions forming the tri-nuclear copper center located at the interface between domain 2 and 3 of each subunit	Cucurbita pepo	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 L-ascorbic acid + O2	-	Cucurbita pepo	2 L-dehydroascorbic acid + 2 H2O	-	?

Subunits

Subunits	Comment	Organism
dimer	unfolding studies, pressure-induced and denaturing agents-induced dissociation and unfolding, and the role of dimerization in the folding strategy of a large protein, crystal structure analysis, physico-chemical properties of a molten dimer enzyme, three distinct domains per subunit, sharing a common beta-barrel topology, overview	Cucurbita pepo

Synonyms

Synonyms	Comment	Organism
ascorbate oxidase	-	Cucurbita pepo

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	assay at	Cucurbita pepo

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Cucurbita pepo

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Release 2023.1 (January 2023)