Any feedback?
Literature summary for 1.10.3.3 extracted from
- Purification and characterisation of ascorbate oxidase from flour and immature wheat kernels. (1999), J. Cereal Sci., 30, 245-254.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
L-ascorbate | - |
Triticum aestivum |  |
| 0.53 | - |
D-isoascorbate | - |
Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
2 * 72000, basic enzyme, SDS-PAGE | Triticum aestivum |
| 139000 | - |
basic enzyme, gel filtration | Triticum aestivum |
| 140000 | - |
acidic enzyme, gel filtration | Triticum aestivum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, phenyl Sepharose, Sephacryl S-300, Q-Sepharose, preparative isoelectric focusing, basic and acidic enzyme purified from flour, ammonium sulfate, phenyl Sepharose, Q-Sepharose, Mono S, Superdex 200, basic and acidic enzyme purified from immature kernels | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-ascorbate + O2 | - |
Triticum aestivum | 2 L-dehydroascorbate + 2 H2O | - |
? | |
| D-isoascorbate + O2 | - |
Triticum aestivum | 2-dehydroisoascorbate + H2O | - |
r | |
| D-isoascorbic acid + O2 | - |
Triticum aestivum | 2-dehydroisoascorbate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 72000, basic enzyme, SDS-PAGE | Triticum aestivum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Triticum aestivum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable for 30 min | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
basic enzyme | Triticum aestivum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Triticum aestivum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
