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Literature summary for 1.10.3.3 extracted from
- Properties of ascorbate oxidase isozymes (1973), Phytochemistry, 12, 2127-2132.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| no change in total activity in the presence of 5 M urea, conversion of tetrameric form into monomer with 75% activity in 8 M urea | Cucurbita pepo medullosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Urea | effect on various molecular forms | Cucumis sativus |  |
| Urea | effect on various molecular forms | Cucurbita pepo condensa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.181 | - |
L-ascorbate | spectrophotometric method | Cucumis sativus | |
| 0.2 | - |
L-ascorbate | spectrophotometric method | Cucurbita pepo condensa | |
| 0.98 | - |
L-ascorbate | Warburg method | Cucurbita pepo condensa | |
| 1.125 | - |
L-ascorbate | Warburg method | Cucumis sativus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
1 * 30000, enzyme also exists as dimer and tetramer, SDS-PAGE | Cucumis sativus |
| 30000 | - |
2 * 30000, enzyme exists as monomer, dimer and tetramer, SDS-PAGE | Cucumis sativus |
| 30000 | - |
4 * 30000, enzyme exists as monomer, dimer and tetramer, SDS-PAGE | Cucumis sativus |
| 35000 | - |
1 * 35000, enzyme also exists as tetramer, octamer, dodecamer and polymer, SDS-PAGE | Cucurbita pepo condensa |
| 35000 | - |
4 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE | Cucurbita pepo condensa |
| 35000 | - |
4 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE | Cucurbita pepo medullosa |
| 35000 | - |
8 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE | Cucurbita pepo condensa |
| 35000 | - |
12 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE | Cucurbita pepo condensa |
| 35000 | - |
x * 35000, between 670000 Da and 2000000 Da, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE | Cucurbita pepo condensa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucumis sativus | - |
cucumber, 3 molecular forms: monomer, dimer, tetramer | - |
| Cucurbita pepo condensa | - |
5 molecular forms: monomer, tetramer, octamer, dodecamer, polymer | - |
| Cucurbita pepo medullosa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Cucumis sativus | - |
| fruit | - |
Cucurbita pepo condensa | - |
| fruit | - |
Cucurbita pepo medullosa | - |
| peel | - |
Cucumis sativus | - |
| peel | - |
Cucurbita pepo condensa | - |
| peel | - |
Cucurbita pepo medullosa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-ascorbate + O2 | - |
Cucumis sativus | 2 L-dehydroascorbate + 2 H2O | - |
? | |
| 2 L-ascorbate + O2 | - |
Cucurbita pepo condensa | 2 L-dehydroascorbate + 2 H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30000, enzyme exists as monomer, dimer and tetramer, SDS-PAGE | Cucumis sativus |
| dodecamer | 12 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE | Cucurbita pepo condensa |
| monomer | 1 * 30000, enzyme also exists as dimer and tetramer, SDS-PAGE | Cucumis sativus |
| monomer | 1 * 35000, enzyme also exists as tetramer, octamer, dodecamer and polymer, SDS-PAGE | Cucurbita pepo condensa |
| octamer | 8 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE | Cucurbita pepo condensa |
| polymer | x * 35000, between 670000 Da and 2000000 Da, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE | Cucurbita pepo condensa |
| tetramer | 4 * 30000, enzyme exists as monomer, dimer and tetramer, SDS-PAGE | Cucumis sativus |
| tetramer | 4 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE | Cucurbita pepo condensa |
| tetramer | 4 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE | Cucurbita pepo medullosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Cucurbita pepo medullosa |
| additional information | - |
different molecular forms vary in resistance to heat inactivation: tetramer of squash and dimer of cucumber being most resistant | Cucumis sativus |
| additional information | - |
different molecular forms vary in resistance to heat inactivation: tetramer of squash and dimer of cucumber being most resistant | Cucurbita pepo condensa |
| 40 | 50 | conversion of octamer and heavier forms to a dimer | Cucumis sativus |
| 40 | 50 | conversion of octamer and heavier forms to a dimer | Cucurbita pepo condensa |
| 100 | - |
1 min, all forms of enzyme inactivated | Cucumis sativus |
| 100 | - |
1 min, all forms of enzyme inactivated | Cucurbita pepo condensa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.6 | - |
loss of 80% activity after 30 min at 0°C | Cucurbita pepo medullosa |
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