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Literature summary for 1.10.3.2 extracted from

  • Mtibaa, R.; Barriuso, J.; de Eugenio, L.; Aranda, E.; Belbahri, L.; Nasri, M.; Martinez, M.J.; Mechichi, T.
    Purification and characterization of a fungal laccase from the ascomycete Thielavia sp. and its role in the decolorization of a recalcitrant dye (2018), Int. J. Biol. Macromol., 120, 1744-1751 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Mg2+ 5 mM, 1.05fold activation Thielavia sp.
Mn2+ 5 mM, 1.27fold activation Thielavia sp.

Application

Application Comment Organism
biotechnology the purified enzyme displays greater efficiency in Remazol Brilliant Blue R decolourization (90%) in absence of redox mediator, an important property for biotechnological applications Thielavia sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 200 mM, 66.72% residual activity Thielavia sp.
Cu2+ 200 mM, 65.15% residual activity Thielavia sp.
EDTA 10 mM, 35% inhibition Thielavia sp.
Fe2+ 200 mM, no residual activity Thielavia sp.
Hg2+ 200 mM, no residual activity Thielavia sp.
L-cysteine 0.5 mM, 25% inhibition Thielavia sp.
Mg2+ 200 mM, 81.32% residual activity Thielavia sp.
NaN3 0.5 mM, 85% inhibition Thielavia sp.
SDS 10 mM, 15% inhibition Thielavia sp.
Zn2+ 200 mM, 85.8% residual activity Thielavia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0237
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 22°C, pH 5.0 Thielavia sp.
0.0243
-
2,6-dimethoxyphenol 22°C, pH 5.0 Thielavia sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thielavia sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Thielavia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
SDS-PAGE Thielavia sp.

Organism

Organism UniProt Comment Textmining
Thielavia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
90.57fold purification with a yield of 11.95% Thielavia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Thielavia sp. ?
-
?
2,6-dimethoxyphenol + O2
-
Thielavia sp. ?
-
?
Remazol Brilliant Blue + O2 greater efficiency in Remazol Brilliant Blue decolourization (90%) in absence of redox mediator Thielavia sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Thielavia sp.

Synonyms

Synonyms Comment Organism
TaLac1
-
Thielavia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Thielavia sp.
70
-
substrate: 2,6-dimethoxyphenol Thielavia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
the enzyme shows 1.3fold thermal activation within 360 min of incubation Thielavia sp.
40
-
the enzyme shows 1.3fold thermal activation within 90 min of incubation Thielavia sp.
60
-
6 h, the enzyme retains 50% of its initial activity Thielavia sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.46
-
2,6-dimethoxyphenol 22°C, pH 5.0 Thielavia sp.
4.14
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 22°C, pH 5.0 Thielavia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Thielavia sp.
6
-
substrate: 2,6-dimethoxyphenol Thielavia sp.