Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM, 1.05fold activation | Thielavia sp. | |
Mn2+ | 5 mM, 1.27fold activation | Thielavia sp. |
Application | Comment | Organism |
---|---|---|
biotechnology | the purified enzyme displays greater efficiency in Remazol Brilliant Blue R decolourization (90%) in absence of redox mediator, an important property for biotechnological applications | Thielavia sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 200 mM, 66.72% residual activity | Thielavia sp. | |
Cu2+ | 200 mM, 65.15% residual activity | Thielavia sp. | |
EDTA | 10 mM, 35% inhibition | Thielavia sp. | |
Fe2+ | 200 mM, no residual activity | Thielavia sp. | |
Hg2+ | 200 mM, no residual activity | Thielavia sp. | |
L-cysteine | 0.5 mM, 25% inhibition | Thielavia sp. | |
Mg2+ | 200 mM, 81.32% residual activity | Thielavia sp. | |
NaN3 | 0.5 mM, 85% inhibition | Thielavia sp. | |
SDS | 10 mM, 15% inhibition | Thielavia sp. | |
Zn2+ | 200 mM, 85.8% residual activity | Thielavia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0237 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 22°C, pH 5.0 | Thielavia sp. | |
0.0243 | - |
2,6-dimethoxyphenol | 22°C, pH 5.0 | Thielavia sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thielavia sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates | Thielavia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
SDS-PAGE | Thielavia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thielavia sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
90.57fold purification with a yield of 11.95% | Thielavia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Thielavia sp. | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Thielavia sp. | ? | - |
? | |
Remazol Brilliant Blue + O2 | greater efficiency in Remazol Brilliant Blue decolourization (90%) in absence of redox mediator | Thielavia sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Thielavia sp. |
Synonyms | Comment | Organism |
---|---|---|
TaLac1 | - |
Thielavia sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Thielavia sp. |
70 | - |
substrate: 2,6-dimethoxyphenol | Thielavia sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
the enzyme shows 1.3fold thermal activation within 360 min of incubation | Thielavia sp. |
40 | - |
the enzyme shows 1.3fold thermal activation within 90 min of incubation | Thielavia sp. |
60 | - |
6 h, the enzyme retains 50% of its initial activity | Thielavia sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.46 | - |
2,6-dimethoxyphenol | 22°C, pH 5.0 | Thielavia sp. | |
4.14 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 22°C, pH 5.0 | Thielavia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Thielavia sp. |
6 | - |
substrate: 2,6-dimethoxyphenol | Thielavia sp. |