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Literature summary for 1.10.3.2 extracted from

  • Gogotya, A.; Nnolim, N.E.; Digban, T.O.; Okoh, A.I.; Nwodo, U.U.
    Characterization of a thermostable and solvent-tolerant laccase produced by Streptomyces sp. LAO (2021), Biotechnol. Lett., 43, 1429-1442 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Urea 100 mM, 1.06fold activation Streptomyces sp. LAO

Application

Application Comment Organism
biotechnology robust catalytic efficiency in the presence of organic solvents suggest its industrial and biotechnological application potentials for the sustainable development of green chemistry Streptomyces sp. LAO

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 1 mM, 30 min, 90.15% residual activity Streptomyces sp. LAO
CuSO4 1 mM, 30 min, 90.81% residual activity Streptomyces sp. LAO
EDTA 100 mM, 93.1% residual activity Streptomyces sp. LAO
FeCl2 1 mM, 30 min, 84.47% residual activity Streptomyces sp. LAO
KCl 1 mM, 30 min, 80.25% residual activity Streptomyces sp. LAO
MgCl2 1 mM, 30 min, 89.34% residual activity Streptomyces sp. LAO
NaN3 100 mM, 96.28% residual activity Streptomyces sp. LAO
NiCl2 1 mM, 30 min, 80.65% residual activity Streptomyces sp. LAO
SDS 100 mM, 69.93% residual activity Streptomyces sp. LAO
ZnSO4 1 mM, 30 min, 84.52% residual activity Streptomyces sp. LAO

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptomyces sp. LAO
-
-

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 20%, 75.9% residual activity after 16 h of preincubation Streptomyces sp. LAO
Ethanol 20%, 82.3% residual activity after 16 h of preincubation Streptomyces sp. LAO
Methanol 20%, 85.12% residual activity after 16 h of preincubation Streptomyces sp. LAO
propanol 20%, 79.1% residual activity after 16 h of preincubation Streptomyces sp. LAO

Organism

Organism UniProt Comment Textmining
Streptomyces sp. LAO MK290990.1
-
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Streptomyces sp. LAO

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 100 30°C: about 70% of maximal activity, 100°C: about 90% of maximal activity Streptomyces sp. LAO

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
after 90 min enzyme activity increases to 138.76% and subsequently decreases, displaying residual activity of 69.90% at 270 min Streptomyces sp. LAO

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Streptomyces sp. LAO

pH Range

pH Minimum pH Maximum Comment Organism
2 10 pH 2.0: about 70% of maximal activity, pH 10.0: about 80% of maximal activity Streptomyces sp. LAO

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
180 min, 66.45% residual activity Streptomyces sp. LAO
7
-
180 min, 102.02% residual activity Streptomyces sp. LAO
8
-
180 min, 119.53% residual activity Streptomyces sp. LAO
9
-
180 min, 86.45% residual activity Streptomyces sp. LAO
10
-
180 min, 82.84% residual activity Streptomyces sp. LAO