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Literature summary for 1.10.3.2 extracted from

  • Hao, J.; Zhang, W.; Wang, H.; Ziya, N.; Luo, Y.; Jia, P.; Zhang, G.; Ng, T.
    Purification and properties of a laccase from the mushroom Agaricus sinodeliciosus (2021), Biotechnol. Appl. Biochem., 68, 297-306 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 10 mM, 3% inhibition Agaricus sinodeliciosus
Ba2+ 10 mM, 16% inhibition Agaricus sinodeliciosus
Ca2+ 10 mM, 6% inhibition Agaricus sinodeliciosus
Cd2+ 10 mM, 81% inhibition Agaricus sinodeliciosus
Cr2+ 10 mM, 5% inhibition Agaricus sinodeliciosus
Cu2+ 10 mM, 4% inhibition Agaricus sinodeliciosus
Fe3+ 10 mM, 29% inhibition Agaricus sinodeliciosus
Hg2+ 10 mM, 79% inhibition Agaricus sinodeliciosus
K+ 10 mM, 26% inhibition Agaricus sinodeliciosus
Na+ 10 mM, 11% inhibition Agaricus sinodeliciosus
Pb2+ 10 mM, 9% inhibition Agaricus sinodeliciosus
Zn2+ 10 mM, 6% inhibition Agaricus sinodeliciosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 30°C Agaricus sinodeliciosus

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 1.25 mM, 1.2fold activation Agaricus sinodeliciosus
Ba2+ 1.25 mM, 1.2fold activation Agaricus sinodeliciosus
Ca2+ 1.25 mM, 1.1fold activation Agaricus sinodeliciosus
Cd2+ 1.25 mM, 1.2fold activation Agaricus sinodeliciosus
Cr2+ 1.25 mM, 1.2fold activation Agaricus sinodeliciosus
Cu2+ 1.25 mM, 1.3fold activation Agaricus sinodeliciosus
Fe3+ 1.25 mM, 1.3,fold activation Agaricus sinodeliciosus
K+ 1.25 mM, 1.2fold activation Agaricus sinodeliciosus
Mg2+ 1.25 mM, 1.2fold activation Agaricus sinodeliciosus
Na+ 1.25 mM, 1.2fold activation Agaricus sinodeliciosus
Pb2+ 1.25 mM, 1.2fold activation Agaricus sinodeliciosus
Zn2+ 1.25 mM, 1.2fold activation Agaricus sinodeliciosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 SDS-PAGE, gel filtration Agaricus sinodeliciosus

Organism

Organism UniProt Comment Textmining
Agaricus sinodeliciosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Agaricus sinodeliciosus

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
 Agaricus sinodeliciosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1610
-
 pH 4.5, 30°C Agaricus sinodeliciosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Agaricus sinodeliciosus ?
-
 ?
benzidine + O2 22.53% of the activity with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Agaricus sinodeliciosus ?
-
 ?
Eriochrome black T + O2
-
 Agaricus sinodeliciosus ?
-
 ?
Malachite Green + O2
-
 Agaricus sinodeliciosus ?
-
 ?
o-toluidine + O2 42.53% of the activity with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Agaricus sinodeliciosus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Agaricus sinodeliciosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Agaricus sinodeliciosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 20°C: about 60% of maximal activity, 70°C: about 50% of maximal activity Agaricus sinodeliciosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Agaricus sinodeliciosus

pH Range

pH Minimum pH Maximum Comment Organism
3 6 pH 3.0: about 60% of maximal activity, pH 6.0: about 55% of maximal activity Agaricus sinodeliciosus