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Literature summary for 1.10.3.2 extracted from

  • Afreen, S.; Shamsi, T.N.; Baig, M.A.; Ahmad, N.; Fatima, S.; Qureshi, M.I.; Hassan, M.I.; Fatma, T.
    A novel multicopper oxidase (laccase) from cyanobacteria Purification, characterization with potential in the decolorization of anthraquinonic dye (2017), PLoS ONE, 12, e0175144 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.10.3.2

Application

Application Comment Organism
degradation cyanobacterial laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. Due to phototrophic mode of nutrition, short generation time and easy mass cultivation, Spirulina platensis laccase appears as good candidate for laccase production. The high yield of laccase in short production period are profitable for its industrial application. Pure Spirulina platensis laccase alone can efficiently decolorized anthraquinonic dye Reactive Blue 4 without any mediators which makes it cost effective and suitable candidate for decolorization of synthetic dyes and help in waste water treatment Arthrospira platensis
environmental protection cyanobacterial laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. Due to phototrophic mode of nutrition, short generation time and easy mass cultivation, Spirulina platensis laccase appears as good candidate for laccase production. The high yield of laccase in short production period are profitable for its industrial application. Pure Spirulina platensis laccase alone can efficiently decolorized anthraquinonic dye Reactive Blue 4 without any mediators which makes it cost effective and suitable candidate for decolorization of synthetic dyes and help in waste water treatment Arthrospira platensis

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM, 79%% inhibition Arthrospira platensis
EDTA 1 mM, 31% inhibition Arthrospira platensis
Hg2+ 1 mM, 4% inhibition Arthrospira platensis
L-cysteine 1 mM, 95% inhibition Arthrospira platensis
Mg2+ 1 mM, 88% inhibition Arthrospira platensis
Sodium azide 1 mM, 98% inhibition Arthrospira platensis
thioglycolic acid 1 mM, 82% inhibition Arthrospira platensis
Thiourea 1 mM, 60% inhibition Arthrospira platensis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Arthrospira platensis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 1 mM, enhances activity to 137% of control Arthrospira platensis
Mn2+ 1 mM, enhances activity to 103% of control Arthrospira platensis
Zn2+ 1 mM, enhances activity to 120% of control Arthrospira platensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 non-denaturing PAGE Arthrospira platensis

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile 10% (v/v), 15 min, 91.8% activity compared to control Arthrospira platensis
dimethylformamide 10% (v/v), 15 min, 58.8% activity compared to control Arthrospira platensis
DMSO 10% (v/v), 15 min, 42.3% activity compared to control Arthrospira platensis
Ethanol 10% (v/v), 15 min, 96.36% activity compared to control Arthrospira platensis
Methanol 10% (v/v), 15 min, 82.5% activity compared to control Arthrospira platensis

Organism

Organism UniProt Comment Textmining
Arthrospira platensis
-
-
-
Arthrospira platensis CFTRI
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arthrospira platensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Arthrospira platensis ?
-
 ?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Arthrospira platensis CFTRI ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 66000, SDS-PAGE Arthrospira platensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Arthrospira platensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 50% of maximal activity, 60°C: about 55% of maximal activioty, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Arthrospira platensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 60 min, enzyme retains 100% of its activity Arthrospira platensis
40
-
 60 min, enzyme retains 97% of its activity Arthrospira platensis
50
-
 30 min, enzyme retains 85.4% of its activity. 90 min, enzyme retains 80% of its activity Arthrospira platensis
60
-
 30 min, enzyme retains 54.0% of its activity. 90 min, enzyme retains 48% of its activity Arthrospira platensis
70
-
 30 min, enzyme retains 15.0% of its activity. 90 min, enzyme retains 10% of its activity. 180 min, complete inactivation Arthrospira platensis
80
-
 90 min, complete inactivation Arthrospira platensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Arthrospira platensis

pH Range

pH Minimum pH Maximum Comment Organism
2 5 pH 2.0: about 75% of maximal activity, pH 5.0: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Arthrospira platensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 1 h, enzyme retains 100% of its activity Arthrospira platensis