Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, 11% loss of activity | Bacillus sp. (in: Bacteria) | |
EDTA | 1 mM, 15.6% loss of activity | Bacillus sp. (in: Bacteria) | |
L-cysteine | 1 mM, reduced 87% of its original activity | Bacillus sp. (in: Bacteria) | |
additional information | the enzyme can tolerate up to 600-800 mM NaCl. NaN3 (1 mM) has almost no effect on the laccase activity | Bacillus sp. (in: Bacteria) | |
SDS | 1 mM, 38.6% loss of activity | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
syringaldazine | pH 8.0, 22°C | Bacillus sp. (in: Bacteria) | |
0.1327 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 22°C | Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | up to 400 mM NaCl stimulates laccase activity. At 600 to 800 mM NaCl, the laccase retains all of its original activity and at higher concentrations, the activity decreases | Bacillus sp. (in: Bacteria) | |
NaCl | halotolerant with maximum activity at 100 mM NaCl | Bacillus sp. (in: Bacteria) | |
Ni2+ | 1 mM, 12% increase in activity | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) WT | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
substrate: syringaldazine, pH 8.0, 22°C | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
2,6-dimethoxyphenol + O2 | 75.8% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
alpha-naphthol + O2 | 22.0% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
catechol + O2 | 27.5% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
catechol + O2 | 27.5% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) WT | ? | - |
? | |
guaiacol + O2 | 90.6% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
L-DOPA + O2 | 13.7% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | decolorization of azo dyes: the enzyme decolorizes sulphonyl green BLE up to 97% at pH 7.0 after 2 h incubation at 35°C, without any addition of mediators. No activity with resorcinol and tyrosine | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | decolorization of azo dyes: the enzyme decolorizes sulphonyl green BLE up to 97% at pH 7.0 after 2 h incubation at 35°C, without any addition of mediators. No activity with resorcinol and tyrosine | Bacillus sp. (in: Bacteria) WT | ? | - |
? | |
pyrogallol + O2 | 95.2% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
pyrogallol + O2 | 95.2% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) WT | ? | - |
? | |
syringaldazine + O2 | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
syringaldazine + O2 | - |
Bacillus sp. (in: Bacteria) WT | ? | - |
? | |
tannic acid + O2 | 17.9% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
tannic acid + O2 | 17.9% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) WT | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 180000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) or syringaldazine | Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | 50°C: about 20% of maximal activity, 90°C: about 40% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
a significant increase in activity is observed until 60 min, in which the relative activity reaches to 150% | Bacillus sp. (in: Bacteria) |
60 | - |
after 15 min incubation relative activity reaches to 140% | Bacillus sp. (in: Bacteria) |
70 | - |
after 15 min incubation relative activity reaches to 148% | Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
51 | - |
syringaldazine | pH 5.0, 22°C | Bacillus sp. (in: Bacteria) | |
309 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 22°C | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) |
8 | - |
substrate: syringaldazine | Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | pH 4.0: about 70% of maximal activity, pH 6.0: about 85% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2328 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 22°C | Bacillus sp. (in: Bacteria) | |
13783 | - |
syringaldazine | pH 8.0, 22°C | Bacillus sp. (in: Bacteria) |