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Literature summary for 1.10.3.2 extracted from

  • Siroosi, M.; Amoozegar, M.; Khajeh, K.
    Purification and characterization of an alkaline chloride-tolerant laccase from a halotolerant bacterium, Bacillus sp. strain WT (2016), J. Mol. Catal. B, 134, 89-97 .
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 1 mM, 11% loss of activity Bacillus sp. (in: Bacteria)
EDTA 1 mM, 15.6% loss of activity Bacillus sp. (in: Bacteria)
L-cysteine 1 mM, reduced 87% of its original activity Bacillus sp. (in: Bacteria)
additional information the enzyme can tolerate up to 600-800 mM NaCl. NaN3 (1 mM) has almost no effect on the laccase activity Bacillus sp. (in: Bacteria)
SDS 1 mM, 38.6% loss of activity Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
syringaldazine pH 8.0, 22°C Bacillus sp. (in: Bacteria)
0.1327
-
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 22°C Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ up to 400 mM NaCl stimulates laccase activity. At 600 to 800 mM NaCl, the laccase retains all of its original activity and at higher concentrations, the activity decreases Bacillus sp. (in: Bacteria)
NaCl halotolerant with maximum activity at 100 mM NaCl Bacillus sp. (in: Bacteria)
Ni2+ 1 mM, 12% increase in activity Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) WT
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
substrate: syringaldazine, pH 8.0, 22°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Bacillus sp. (in: Bacteria) ?
-
?
2,6-dimethoxyphenol + O2 75.8% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria) ?
-
?
alpha-naphthol + O2 22.0% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria) ?
-
?
catechol + O2 27.5% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria) ?
-
?
catechol + O2 27.5% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria) WT ?
-
?
guaiacol + O2 90.6% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria) ?
-
?
L-DOPA + O2 13.7% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria) ?
-
?
additional information decolorization of azo dyes: the enzyme decolorizes sulphonyl green BLE up to 97% at pH 7.0 after 2 h incubation at 35°C, without any addition of mediators. No activity with resorcinol and tyrosine Bacillus sp. (in: Bacteria) ?
-
?
additional information decolorization of azo dyes: the enzyme decolorizes sulphonyl green BLE up to 97% at pH 7.0 after 2 h incubation at 35°C, without any addition of mediators. No activity with resorcinol and tyrosine Bacillus sp. (in: Bacteria) WT ?
-
?
pyrogallol + O2 95.2% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria) ?
-
?
pyrogallol + O2 95.2% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria) WT ?
-
?
syringaldazine + O2
-
Bacillus sp. (in: Bacteria) ?
-
?
syringaldazine + O2
-
Bacillus sp. (in: Bacteria) WT ?
-
?
tannic acid + O2 17.9% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria) ?
-
?
tannic acid + O2 17.9% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria) WT ?
-
?

Subunits

Subunits Comment Organism
? x * 180000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) or syringaldazine Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 50°C: about 20% of maximal activity, 90°C: about 40% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
a significant increase in activity is observed until 60 min, in which the relative activity reaches to 150% Bacillus sp. (in: Bacteria)
60
-
after 15 min incubation relative activity reaches to 140% Bacillus sp. (in: Bacteria)
70
-
after 15 min incubation relative activity reaches to 148% Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
51
-
syringaldazine pH 5.0, 22°C Bacillus sp. (in: Bacteria)
309
-
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 22°C Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria)
8
-
substrate: syringaldazine Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
4 6 pH 4.0: about 70% of maximal activity, pH 6.0: about 85% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria)

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2328
-
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 22°C Bacillus sp. (in: Bacteria)
13783
-
syringaldazine pH 8.0, 22°C Bacillus sp. (in: Bacteria)