Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 1.27fold activation | Bacillus licheniformis |
Cloned (Comment) | Organism |
---|---|
heterologously expressed in Escherichia coli | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 1 mM, complete inactivation | Bacillus licheniformis | |
L-cysteine | 1 mM, complete inactivation | Bacillus licheniformis | |
NaN3 | 1 mM, 60% inhibition | Bacillus licheniformis |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | presence of 10% organic solvent reduces melting temperature from 79°C to to 45-52°C but activity remains practically unimpaired | Bacillus licheniformis |
dimethyl formamide | presence of 10% organic solvent reduces melting temperature from 79°C to to 45-52°C but activity remains practically unimpaired | Bacillus licheniformis |
dimethyl sulfoxide | presence of 10% organic solvent reduces melting temperature from 79°C to to 45-52°C but activity remains practically unimpaired | Bacillus licheniformis |
Methanol | presence of 10% organic solvent reduces melting temperature from 79°C to to 45-52°C but activity remains practically unimpaired | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Bacillus licheniformis ATCC 9945a | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | 73% of the activity compared to syringaldazine | Bacillus licheniformis | ? | - |
? | |
2,6-dimethoxyphenol + O2 | 69% of the activity compared to syringaldazine | Bacillus licheniformis | ? | - |
? | |
catechol + O2 | 28% of the activity compared to syringaldazine | Bacillus licheniformis | ? | - |
? | |
catechol + O2 | 28% of the activity compared to syringaldazine | Bacillus licheniformis ATCC 9945a | ? | - |
? | |
gallic acid + O2 | 45% of the activity compared to syringaldazine | Bacillus licheniformis | ? | - |
? | |
gallic acid + O2 | 45% of the activity compared to syringaldazine | Bacillus licheniformis ATCC 9945a | ? | - |
? | |
L-DOPA + O2 | 35% of the activity compared to syringaldazine | Bacillus licheniformis | ? | - |
? | |
additional information | the enzyme mediates C-C bond formation via oxidative coupling after one electron oxidation of phenolic group. Successful polymerization of 2-naphthol is achieved with 77% conversion of 250 mg/l 2-naphtol in 15 min | Bacillus licheniformis | ? | - |
? | |
additional information | the enzyme mediates C-C bond formation via oxidative coupling after one electron oxidation of phenolic group. Successful polymerization of 2-naphthol is achieved with 77% conversion of 250 mg/l 2-naphtol in 15 min | Bacillus licheniformis ATCC 9945a | ? | - |
? | |
o-dianisidine + O2 | 90% of the activity compared to syringaldazine | Bacillus licheniformis | ? | - |
? | |
p-phenylenediamine + O2 | 107% of the activity compared to syringaldazine | Bacillus licheniformis | ? | - |
? | |
p-phenylenediamine + O2 | 107% of the activity compared to syringaldazine | Bacillus licheniformis ATCC 9945a | ? | - |
? | |
syringaldazine + O2 | - |
Bacillus licheniformis | ? | - |
? | |
syringaldazine + O2 | - |
Bacillus licheniformis ATCC 9945a | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 52 | melting temperature in presence of 10% organic solvents such as acetonitrile, dimethylformamide, dimethylsulfoxide or methanol | Bacillus licheniformis |
70 | - |
half-life: 50 min | Bacillus licheniformis |
79 | - |
melting temperature at pH 7.0. Presence of 10% organic solvents such as acetonitrile, dimethylformamide, dimethylsulfoxide or methanol reduces melting temperature to 45-52°C | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus licheniformis |
7 | - |
substrate: syringaldazine | Bacillus licheniformis |