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Literature summary for 1.10.3.2 extracted from

  • Loncar, N.; Bozic, N.; Vujcic, Z.
    Expression and characterization of a thermostable organic solvent-tolerant laccase from Bacillus licheniformis ATCC 9945a (2016), J. Mol. Catal. B, 134, 390-395 .
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM, 1.27fold activation Bacillus licheniformis

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Escherichia coli Bacillus licheniformis

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 1 mM, complete inactivation Bacillus licheniformis
L-cysteine 1 mM, complete inactivation Bacillus licheniformis
NaN3 1 mM, 60% inhibition Bacillus licheniformis

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile presence of 10% organic solvent reduces melting temperature from 79°C to to 45-52°C but activity remains practically unimpaired Bacillus licheniformis
dimethyl formamide presence of 10% organic solvent reduces melting temperature from 79°C to to 45-52°C but activity remains practically unimpaired Bacillus licheniformis
dimethyl sulfoxide presence of 10% organic solvent reduces melting temperature from 79°C to to 45-52°C but activity remains practically unimpaired Bacillus licheniformis
Methanol presence of 10% organic solvent reduces melting temperature from 79°C to to 45-52°C but activity remains practically unimpaired Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-
Bacillus licheniformis ATCC 9945a
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 73% of the activity compared to syringaldazine Bacillus licheniformis ?
-
?
2,6-dimethoxyphenol + O2 69% of the activity compared to syringaldazine Bacillus licheniformis ?
-
?
catechol + O2 28% of the activity compared to syringaldazine Bacillus licheniformis ?
-
?
catechol + O2 28% of the activity compared to syringaldazine Bacillus licheniformis ATCC 9945a ?
-
?
gallic acid + O2 45% of the activity compared to syringaldazine Bacillus licheniformis ?
-
?
gallic acid + O2 45% of the activity compared to syringaldazine Bacillus licheniformis ATCC 9945a ?
-
?
L-DOPA + O2 35% of the activity compared to syringaldazine Bacillus licheniformis ?
-
?
additional information the enzyme mediates C-C bond formation via oxidative coupling after one electron oxidation of phenolic group. Successful polymerization of 2-naphthol is achieved with 77% conversion of 250 mg/l 2-naphtol in 15 min Bacillus licheniformis ?
-
?
additional information the enzyme mediates C-C bond formation via oxidative coupling after one electron oxidation of phenolic group. Successful polymerization of 2-naphthol is achieved with 77% conversion of 250 mg/l 2-naphtol in 15 min Bacillus licheniformis ATCC 9945a ?
-
?
o-dianisidine + O2 90% of the activity compared to syringaldazine Bacillus licheniformis ?
-
?
p-phenylenediamine + O2 107% of the activity compared to syringaldazine Bacillus licheniformis ?
-
?
p-phenylenediamine + O2 107% of the activity compared to syringaldazine Bacillus licheniformis ATCC 9945a ?
-
?
syringaldazine + O2
-
Bacillus licheniformis ?
-
?
syringaldazine + O2
-
Bacillus licheniformis ATCC 9945a ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 52 melting temperature in presence of 10% organic solvents such as acetonitrile, dimethylformamide, dimethylsulfoxide or methanol Bacillus licheniformis
70
-
half-life: 50 min Bacillus licheniformis
79
-
melting temperature at pH 7.0. Presence of 10% organic solvents such as acetonitrile, dimethylformamide, dimethylsulfoxide or methanol reduces melting temperature to 45-52°C Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus licheniformis
7
-
substrate: syringaldazine Bacillus licheniformis