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Literature summary for 1.10.3.2 extracted from

  • Bagewadi, Z.; Mulla, S.; Ninnekar, H.
    Purification and immobilization of laccase from Trichoderma harzianum strain HZN10 and its application in dye decolorization (2017), J. Genet. Eng. Biotechnol., 15, 139-150 .
No PubMed abstract available
Search for more literature for 1.10.3.2

Activating Compound

Activating Compound Comment Organism Structure
glycerol 20% (v/v), 15% increase inactivity Trichoderma harzianum
Tween-40 0.5% (v/v), 10% increase in activity Trichoderma harzianum

Application

Application Comment Organism
degradation laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment Trichoderma harzianum
environmental protection laccase can be efficiently used to decolorize synthetic dye and is a suitable candidate for the treatment of wastewater from industrial effluents. The wide pH- and thermostability attributes of immobilized laccase make them suitable for environmental applications Trichoderma harzianum

General Stability

General Stability Organism
Ca-alginate, Cu-alginate, Ca-alginate-chitosan and solĀ–gel immobilized laccase show an operational stability of 36%, 51%, 66% and 82%, respectively after 6 cycles Trichoderma harzianum
soluble enzyme loses 45% of its activity after 8 days at 4Ā°C. Sol-gel matrix immobilized laccase is the most stable with a mere loss of 2% among the Ca-alginate-chitosan, Cu-alginate and Ca-alginate beads with a loss of 10%, 17% and 25%, respectively Trichoderma harzianum

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 5 mM, 15% loss of activity Trichoderma harzianum
acetone 20% (v/v), 42% loss of activity Trichoderma harzianum
Al3+ 5 mM, 60% loss of activity Trichoderma harzianum
Cd2+ 5 mM, 78% loss of activity Trichoderma harzianum
Co2+ 5 mM, 67% loss of activity Trichoderma harzianum
dithiothreitol 5 mM, 65% loss of activity Trichoderma harzianum
EDTA 5 mM, 70% loss of activity Trichoderma harzianum
ethanol 20% (v/v), 15% loss of activity Trichoderma harzianum
Fe2+ 5 mM, 60% loss of activity Trichoderma harzianum
Hg2+ 5 mM, 90% loss of activity Trichoderma harzianum
Isopropanol 20% (v/v), 30% loss of activity Trichoderma harzianum
L-cysteine 5 mM, 20% loss of activity Trichoderma harzianum
methanol 20% (v/v), 20% loss of activity Trichoderma harzianum
additional information soluble laccase shows more than 80% relative activity in organic solvents like ethanol, methanol, toluene and acetonitrile Trichoderma harzianum
Ni2+ 5 mM, 35% loss of activity Trichoderma harzianum
SDS 0.5%, 60% loss of activity Trichoderma harzianum
Toluene 20% (v/v), 18% loss of activity Trichoderma harzianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 6, 25Ā°C, soluble enzyme Trichoderma harzianum
2
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 6, 25Ā°C,laccase immobilized in solĀ–gel matrix Trichoderma harzianum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Trichoderma harzianum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, 18% increase in activity Trichoderma harzianum
Cu2+ 5 mM, 85% increase in activity Trichoderma harzianum
Mg2+ 5 mM, 15% increase in activity Trichoderma harzianum
Mn2+ 5 mM, 8% increase in activity Trichoderma harzianum

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum
-
-
-
Trichoderma harzianum HZN10
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma harzianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
164
-
 pH 6, 25Ā°C Trichoderma harzianum

Storage Stability

Storage Stability Organism
4Ā°C, 8 days, soluble enzyme loses 45% of its activity. SolĀ–gel matrix immobilized laccase is the most stable with a mere loss of 2% among the Ca-alginate-chitosan, Cu-alginate and Ca-alginate beads with a loss of 10%, 17% and 25%, respectively Trichoderma harzianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trichoderma harzianum ?
-
 ?
2,6-dimethoxyphenol + O2 96% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum ?
-
 ?
catechol + O2 44% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum ?
-
 ?
catechol + O2 44% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum HZN10 ?
-
 ?
ferulic acid + O2 24% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum ?
-
 ?
ferulic acid + O2 24% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum HZN10 ?
-
 ?
gallic acid + O2 54% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum ?
-
 ?
gallic acid + O2 54% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum HZN10 ?
-
 ?
guaiacol + O2 83% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum ?
-
 ?
additional information free and immobilized laccase is employed for decolorization of three different synthetic dyes (malachite green, methylene blue and congo red). Approximately 100% of malachite green, 90% of methylene blue and 60% of congo red dyes at initial concentration of 200 mg/l are decolorized within 16, 18 and 20 h, respectively by laccase immobilized in sol-gel matrix in the presence of 1-hydroxybenzotriazole mediator Trichoderma harzianum ?
-
 ?
additional information free and immobilized laccase is employed for decolorization of three different synthetic dyes (malachite green, methylene blue and congo red). Approximately 100% of malachite green, 90% of methylene blue and 60% of congo red dyes at initial concentration of 200 mg/l are decolorized within 16, 18 and 20 h, respectively by laccase immobilized in sol-gel matrix in the presence of 1-hydroxybenzotriazole mediator Trichoderma harzianum HZN10 ?
-
 ?
syringaldazine + O2 148% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum ?
-
 ?
syringaldazine + O2 148% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum HZN10 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Trichoderma harzianum

Temperature Optimum [Ā°C]

Temperature Optimum [Ā°C] Temperature Optimum Maximum [Ā°C] Comment Organism
50
-
 soluble and immobilized laccases Trichoderma harzianum

Temperature Range [Ā°C]

Temperature Minimum [Ā°C] Temperature Maximum [Ā°C] Comment Organism
50 70 all the forms of immobilized laccase show more than 90% relative activity at higher temperature of 55Ā°C and 60Ā°C. Sol-gel immobilized laccase shows 97% (65Ā°C) and 94% (70Ā°C) relative activity at higher temperatures. Soluble enzyme loses maximum activity above 55Ā°C Trichoderma harzianum

Temperature Stability [Ā°C]

Temperature Stability Minimum [Ā°C] Temperature Stability Maximum [Ā°C] Comment Organism
50
-
 4 h, soluble and immobilized laccases retain more than 90% activity at 50Ā°C. Copper alginate shows better stability than calcium alginate immobilized laccase Trichoderma harzianum
55
-
 4 h, calcium alginate-chitosan immobilized laccase retains 91% activity Trichoderma harzianum
65
-
 4 h, sol-gel immobilized laccase retains 90% activity Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 7 laccase immobilized in sol-gel matrix Trichoderma harzianum
5 6 copper alginate and calcium alginate-chitosan immobilized laccase Trichoderma harzianum
6
-
 soluble enzyme and calcium alginate immobilized enzyme Trichoderma harzianum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 4 h, solĀ–gel immobilized laccase retains 98% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀ–gel matrix > calcium alginateĀ–chitosan > copper alginate > calcium alginate Trichoderma harzianum
5 6 4 h, solĀ–gel immobilized laccase retains 100% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀ–gel matrix > calcium alginateĀ–chitosan > copper alginate > calcium alginate Trichoderma harzianum
7
-
 4 h, solĀ–gel immobilized laccase retains 96% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀ–gel matrix > calcium alginateĀ–chitosan > copper alginate > calcium alginate Trichoderma harzianum
8
-
 4 h, solĀ–gel immobilized laccase retains 92% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀ–gel matrix > calcium alginateĀ–chitosan > copper alginate > calcium alginate Trichoderma harzianum