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Literature summary for 1.10.3.2 extracted from

  • Chmelova, D.; Ondrejovic, M.
    Purification and characterization of extracellular laccase produced by Ceriporiopsis subvermispora and decolorization of triphenylmethane dyes (2016), J. Basic Microbiol., 56, 1173-1182 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Activating Compound

Activating Compound Comment Organism Structure
EDTA 10 mM, 1.8fold activation Gelatoporia subvermispora
SDS 1.6fold activation at 1 mM, 89% inhibition at 10 mM Gelatoporia subvermispora

Application

Application Comment Organism
degradation degradation of synthetic dyes from wastewater using biological treatment Gelatoporia subvermispora
environmental protection degradation of synthetic dyes from wastewater using biological treatment Gelatoporia subvermispora

Inhibitors

Inhibitors Comment Organism Structure
K+ 1 mM, 4% inhibition, 10 mM, 15% inhibition Gelatoporia subvermispora
Li+ 1 mM, 19% inhibition Gelatoporia subvermispora
Na+ 1 mM, 4% inhibition Gelatoporia subvermispora
SDS 1.6fold activation at 1 mM, 89% inhibition at 10 mM Gelatoporia subvermispora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) 22°C, pH 5.0 Gelatoporia subvermispora

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Gelatoporia subvermispora
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 10 mM, 1.6fold activation Gelatoporia subvermispora
Ca2+ 10 mM, 1.2fold activation Gelatoporia subvermispora
Cu2+ 10 mM, 8.0fold activation Gelatoporia subvermispora
Fe2+ 10 mM, 2.6fold activation Gelatoporia subvermispora
Mg2+ 10 mM, 1.5fold activation Gelatoporia subvermispora
Mn2+ 10 mM, 2.7fold activation Gelatoporia subvermispora
Sn2+ 10 mM, 6.1fold activation Gelatoporia subvermispora
Zn2+ 10 mM, 6.4fold activation Gelatoporia subvermispora

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 SDS_PAGE Gelatoporia subvermispora

Organism

Organism UniProt Comment Textmining
Gelatoporia subvermispora
-
-
-
Gelatoporia subvermispora ATCC90467
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gelatoporia subvermispora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Gelatoporia subvermispora ?
-
 ?
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Gelatoporia subvermispora ATCC90467 ?
-
 ?
Bromocresol Purple + O2 the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator Gelatoporia subvermispora ?
-
 ?
Bromocresol Purple + O2 the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator Gelatoporia subvermispora ATCC90467 ?
-
 ?
Malachite Green + O2 the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator Gelatoporia subvermispora ?
-
 ?
Malachite Green + O2 the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator Gelatoporia subvermispora ATCC90467 ?
-
 ?
Methyl Violet + O2 the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator Gelatoporia subvermispora ?
-
 ?
Methyl Violet + O2 the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator Gelatoporia subvermispora ATCC90467 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Gelatoporia subvermispora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Gelatoporia subvermispora

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
 50 h, about 55% loss of activity Gelatoporia subvermispora
30
-
 40 h, 70% loss of activity Gelatoporia subvermispora
40
-
 80 h, about 50% loss of activity Gelatoporia subvermispora
50
-
 20 h, 80% loss of activity Gelatoporia subvermispora
60
-
 unstable at Gelatoporia subvermispora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2
-
-
 Gelatoporia subvermispora