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Literature summary for 1.10.3.2 extracted from

  • Feng, H.; Zhang, D.; Sun, Y.; Zhi, Y.; Mao, L.; Luo, Y.; Xu, L.; Wang, L.; Zhou, P.
    Expression and characterization of a recombinant laccase with alkalistable and thermostable properties from Streptomyces griseorubens JSD-1 (2015), Appl. Biochem. Biotechnol., 176, 547-562 .
    View publication on PubMed

Application

Application Comment Organism
degradation laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. The enzyme alone can decolourize indigo carmine partially after 60-min incubation at 45 °C. Decolorization is much more efficient in the presence of syringaldehyde. Nearly 90 % decolorization is observed within 20 min Streptomyces griseorubens
environmental protection laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. The enzyme alone can decolorize indigo carmine partially after 60-min incubation at 45°C. Decolorization is much more efficient in the presence of syringaldehyde. Nearly 90 % decolorization is observed within 20 min Streptomyces griseorubens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces griseorubens

Inhibitors

Inhibitors Comment Organism Structure
CdCl2 1 mM, 24.6% inhibition Streptomyces griseorubens
CoCl2 1 mM, 20.8% inhibition Streptomyces griseorubens
dithiothreitol 1 mM, 10% loss of activity Streptomyces griseorubens
EDTA 1 mM, 62% loss of activity Streptomyces griseorubens
HgCl2 1 mM, 33.3% inhibition Streptomyces griseorubens
NaN3 1 mM, 5.5% loss of activity Streptomyces griseorubens
SDS 1 mM, 20% loss of activity Streptomyces griseorubens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptomyces griseorubens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
CuCl2 1 mM, increases activity by 28.5% Streptomyces griseorubens
MnCl2 1 mM, increases activity by 11.7% Streptomyces griseorubens

Organism

Organism UniProt Comment Textmining
Streptomyces griseorubens
-
-
-
Streptomyces griseorubens JSD-1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Streptomyces griseorubens ?
-
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Streptomyces griseorubens JSD-1 ?
-
?
2,6-dimethoxyphenol + O2
-
Streptomyces griseorubens ?
-
?
2,6-dimethoxyphenol + O2
-
Streptomyces griseorubens JSD-1 ?
-
?
guaiacol + O2
-
Streptomyces griseorubens ?
-
?
guaiacol + O2
-
Streptomyces griseorubens JSD-1 ?
-
?
syringaldazine + O2
-
Streptomyces griseorubens ?
-
?
syringaldazine + O2
-
Streptomyces griseorubens JSD-1 ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
8 h, 40% loss of activity Streptomyces griseorubens
40
-
8 h, 60% loss of activity Streptomyces griseorubens
50
-
8 h, 70% loss of activity Streptomyces griseorubens
60
-
8 h, 90% loss of activity Streptomyces griseorubens
70
-
8 h, complete loss of activity Streptomyces griseorubens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.058
-
2,6-dimethoxyphenol 25°C, pH 9.0 Streptomyces griseorubens
0.337
-
syringaldazine 25°C, pH 8.0 Streptomyces griseorubens
7.7
-
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) 25°C, pH 4.0 Streptomyces griseorubens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Streptomyces griseorubens
8
-
substrate: syringaldazine Streptomyces griseorubens
9
-
substrate: 2,6-dimethoxyphenol Streptomyces griseorubens
10
-
substrate: guaiacol Streptomyces griseorubens

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
complete loss of activity within 1 day Streptomyces griseorubens
4 5 enzyme loses 50% activity within 2 days Streptomyces griseorubens
7 11 the enzyme retains over 50% residual activity after 5-day incubation Streptomyces griseorubens
10
-
highest stability is observed at pH 10.0 with an approximately residual activity of 90% after 5 days Streptomyces griseorubens

Expression

Organism Comment Expression
Streptomyces griseorubens expression levels were are upregulated with the stimulation of rice straw or lignin. However, it iss expressed in relative low levels when induced by cellulose, xylan, pectin or glucose up