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Literature summary for 1.10.3.2 extracted from

  • Xu, Y.; Lu, Y.; Zhang, R.; Wang, H.; Liu, Q.
    Characterization of a novel laccase purified from the fungus Hohenbuehelia serotina and its decolourisation of dyes (2016), Acta Biochim. Pol., 63, 273-279 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Application

Application Comment Organism
degradation laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment Sarcomyxa serotina
environmental protection laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment Sarcomyxa serotina

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 12.5 mM, complete inhibition Sarcomyxa serotina
Al3+ 10 mM, 43% loss of activity Sarcomyxa serotina
Ca2+ 10 mM, 35% loss of activity Sarcomyxa serotina
Fe2+ 10 mM, 72.4% loss of activity Sarcomyxa serotina
K+ 10 mM, 7% loss of activity Sarcomyxa serotina
L-cysteine 50 mM, 15% inhibition Sarcomyxa serotina
Mg2+ 10 mM, 38% loss of activity Sarcomyxa serotina
Mn2+ 10 mM, 38% loss of activity Sarcomyxa serotina
additional information EDTA and DMSO have almost no inhibitory effect Sarcomyxa serotina
SDS 3 mM, 85% inhibition Sarcomyxa serotina

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ significantly enhances activity at concentrations under 50 mM Sarcomyxa serotina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 gel filtration Sarcomyxa serotina

Organism

Organism UniProt Comment Textmining
Sarcomyxa serotina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sarcomyxa serotina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Sarcomyxa serotina ?
-
 ?
2-methylcatechol + O2 17.2% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sarcomyxa serotina ?
-
 ?
N,N-dimethyl-1,4-phenylenediamine + O2 42.6% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sarcomyxa serotina ?
-
 ?
pyrogallol + O2 7.7% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sarcomyxa serotina ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 57000, SDS-PAGE Sarcomyxa serotina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sarcomyxa serotina

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 40% of maximal activity, 50°C: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sarcomyxa serotina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 3 h, at temperatures below 40°C, there is almost no change in enzymatic activity Sarcomyxa serotina
50
-
 3 h, at temperatures over 50°C the remaining laccase activity drops to below 70% of the initial activity Sarcomyxa serotina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sarcomyxa serotina

pH Range

pH Minimum pH Maximum Comment Organism
4 9 pH 4.0: about 65% of maximal activity, pH 9.0: about 70% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sarcomyxa serotina

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.8
-
 2 h, 30% loss of activity Sarcomyxa serotina
6.2
-
 2 h, 27% loss of activity Sarcomyxa serotina
6.5
-
 2 h, 18% loss of activity Sarcomyxa serotina