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Literature summary for 1.10.3.2 extracted from

  • Chefetz, B.; Chen, Y.; Hadar, Y.
    Purification and characterization of laccase from Chaetomium thermophilium and its role in humification (1998), Appl. Environ. Microbiol., 64, 3175-3179.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, 67% inhibition. 10 mM, 95% inhibition Thermochaetoides thermophila
additional information strongly inhibited by Cu-chelating agents Thermochaetoides thermophila
Sodium azide 0.05 mM, 84% inhibition Thermochaetoides thermophila
thioglycolic acid 0.1 mM, 91% inhibition Thermochaetoides thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
syringaldazine pH 6.0, 50°C Thermochaetoides thermophila
0.036
-
hydroxyquinone pH 6.0, 50°C Thermochaetoides thermophila
0.096
-
2,6-dimethoxyphenol pH 6.0, 50°C Thermochaetoides thermophila
0.1
-
L-3,4-dihydroxyphenylalanine pH 6.0, 50°C Thermochaetoides thermophila
0.13
-
gallic acid pH 6.0, 50°C Thermochaetoides thermophila
0.15
-
vanillic acid pH 6.0, 50°C Thermochaetoides thermophila
0.19
-
2,2'-azinobis(3-ethylbenz-thiazoline-6-sulfonic acid) pH 6.0, 50°C Thermochaetoides thermophila
0.27
-
ferulic acid pH 6.0, 50°C Thermochaetoides thermophila
0.4
-
guaiacol pH 6.0, 50°C Thermochaetoides thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
x * 77000, SDS-PAGE Thermochaetoides thermophila

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Thermochaetoides thermophila

Purification (Commentary)

Purification (Comment) Organism
-
Thermochaetoides thermophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37
-
pH 6.0, 50°C Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenz-thiazoline-6-sulfonic acid) + O2
-
Thermochaetoides thermophila ?
-
?
2,6-dimethoxyphenol + O2
-
Thermochaetoides thermophila ?
-
?
ferulic acid + O2
-
Thermochaetoides thermophila ?
-
?
gallic acid + O2
-
Thermochaetoides thermophila ?
-
?
guaiacol + O2
-
Thermochaetoides thermophila ?
-
?
hydroxyquinone + O2
-
Thermochaetoides thermophila ?
-
?
L-3,4-dihydroxyphenylalanine + O2 i.e. L-dopa Thermochaetoides thermophila ?
-
?
additional information no activity with tyrosine Thermochaetoides thermophila ?
-
?
syringaldazine + O2
-
Thermochaetoides thermophila ?
-
?
vanillic acid + O2
-
Thermochaetoides thermophila ?
-
?

Subunits

Subunits Comment Organism
? x * 77000, SDS-PAGE Thermochaetoides thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
Thermochaetoides thermophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C: about 50% of maximal activity, 70°C: about 90% of maximal activity Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
half-life: 24 h Thermochaetoides thermophila
50
-
half-life: 12 h Thermochaetoides thermophila
70
-
1 h, stable Thermochaetoides thermophila
80
-
half-life: 10 min Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
oxidation of syringaldazine Thermochaetoides thermophila

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 20% of maximal activity, pH 8.0: about 65% of maximal activity. The enzyme is inactive at pH 4.0 and at pH 9.0 Thermochaetoides thermophila

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 1 h, stable Thermochaetoides thermophila

pI Value

Organism Comment pI Value Maximum pI Value
Thermochaetoides thermophila isoelectric focusing
-
5.1