Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM, 67% inhibition. 10 mM, 95% inhibition | Thermochaetoides thermophila | |
additional information | strongly inhibited by Cu-chelating agents | Thermochaetoides thermophila | |
Sodium azide | 0.05 mM, 84% inhibition | Thermochaetoides thermophila | |
thioglycolic acid | 0.1 mM, 91% inhibition | Thermochaetoides thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
syringaldazine | pH 6.0, 50°C | Thermochaetoides thermophila | |
0.036 | - |
hydroxyquinone | pH 6.0, 50°C | Thermochaetoides thermophila | |
0.096 | - |
2,6-dimethoxyphenol | pH 6.0, 50°C | Thermochaetoides thermophila | |
0.1 | - |
L-3,4-dihydroxyphenylalanine | pH 6.0, 50°C | Thermochaetoides thermophila | |
0.13 | - |
gallic acid | pH 6.0, 50°C | Thermochaetoides thermophila | |
0.15 | - |
vanillic acid | pH 6.0, 50°C | Thermochaetoides thermophila | |
0.19 | - |
2,2'-azinobis(3-ethylbenz-thiazoline-6-sulfonic acid) | pH 6.0, 50°C | Thermochaetoides thermophila | |
0.27 | - |
ferulic acid | pH 6.0, 50°C | Thermochaetoides thermophila | |
0.4 | - |
guaiacol | pH 6.0, 50°C | Thermochaetoides thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
x * 77000, SDS-PAGE | Thermochaetoides thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermochaetoides thermophila | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Thermochaetoides thermophila |
Purification (Comment) | Organism |
---|---|
- |
Thermochaetoides thermophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37 | - |
pH 6.0, 50°C | Thermochaetoides thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenz-thiazoline-6-sulfonic acid) + O2 | - |
Thermochaetoides thermophila | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Thermochaetoides thermophila | ? | - |
? | |
ferulic acid + O2 | - |
Thermochaetoides thermophila | ? | - |
? | |
gallic acid + O2 | - |
Thermochaetoides thermophila | ? | - |
? | |
guaiacol + O2 | - |
Thermochaetoides thermophila | ? | - |
? | |
hydroxyquinone + O2 | - |
Thermochaetoides thermophila | ? | - |
? | |
L-3,4-dihydroxyphenylalanine + O2 | i.e. L-dopa | Thermochaetoides thermophila | ? | - |
? | |
additional information | no activity with tyrosine | Thermochaetoides thermophila | ? | - |
? | |
syringaldazine + O2 | - |
Thermochaetoides thermophila | ? | - |
? | |
vanillic acid + O2 | - |
Thermochaetoides thermophila | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 77000, SDS-PAGE | Thermochaetoides thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | - |
Thermochaetoides thermophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | 40°C: about 50% of maximal activity, 70°C: about 90% of maximal activity | Thermochaetoides thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
half-life: 24 h | Thermochaetoides thermophila |
50 | - |
half-life: 12 h | Thermochaetoides thermophila |
70 | - |
1 h, stable | Thermochaetoides thermophila |
80 | - |
half-life: 10 min | Thermochaetoides thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
oxidation of syringaldazine | Thermochaetoides thermophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5.0: about 20% of maximal activity, pH 8.0: about 65% of maximal activity. The enzyme is inactive at pH 4.0 and at pH 9.0 | Thermochaetoides thermophila |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | 1 h, stable | Thermochaetoides thermophila |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermochaetoides thermophila | isoelectric focusing | - |
5.1 |