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Literature summary for 1.10.3.2 extracted from

  • Freixo, M.d.o..R.; Karmali, A.; Arteiro, J.M.
    Production, purification and characterization of laccase from Pleurotus ostreatus grown on tomato pomace (2012), World J. Microbiol. Biotechnol., 28, 245-254.
    View publication on PubMed
Search for more literature for 1.10.3.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0172
-
 2-dianisidine pH 6.0, 25°C Pleurotus ostreatus
0.0552
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.0, 25°C Pleurotus ostreatus
4
-
 guaiacol pH 6.0, 25°C Pleurotus ostreatus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the enzyme is a multi-copper enzyme Pleurotus ostreatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 native PAGE and gel filtration Pleurotus ostreatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pleurotus ostreatus the enzyme catalyzes the oxidation of a wide range of compounds, including poly-phenols and anilines. The one electron oxidation of the reducing substrate occurs concomitantly with the four-electron reduction of molecular oxygen to water ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus
-
 grown on tomato pomace, several isoyzmes
-

Purification (Commentary)

Purification (Comment) Organism
method development for one-step affinity chromatographic purifcation, 46fold purification on 1,4-butanediol diglycidyl ether affinity resin, analysis of affinity chromatographic matrices containing either urea, acetamide, ethanolamine, or iminodiacetic acid, and best with 1,4-butanediol diglycidyl ether, as affinity ligands for enzyme purification from fermentation broth Pleurotus ostreatus

Source Tissue

Source Tissue Comment Organism Textmining
additional information cultivation of Pleurotus ostreatus on tomato pomace medium Pleurotus ostreatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.8
-
 purified native enzyme, pH 6.0, 25°C Pleurotus ostreatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Pleurotus ostreatus ?
-
 ?
2-dianisidine + O2
-
 Pleurotus ostreatus ?
-
 ?
guaiacol + O2
-
 Pleurotus ostreatus ?
-
 ?
additional information the enzyme catalyzes the oxidation of a wide range of compounds, including poly-phenols and anilines. The one electron oxidation of the reducing substrate occurs concomitantly with the four-electron reduction of molecular oxygen to water Pleurotus ostreatus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 52000-55000, SDS-PAGE Pleurotus ostreatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pleurotus ostreatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.9
-
 guaiacol pH 6.0, 25°C Pleurotus ostreatus
20
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.0, 25°C Pleurotus ostreatus
20.7
-
 2-dianisidine pH 6.0, 25°C Pleurotus ostreatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Pleurotus ostreatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0172
-
 2-dianisidine pH 6.0, 25°C Pleurotus ostreatus
0.0552
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.0, 25°C Pleurotus ostreatus
4
-
 guaiacol pH 6.0, 25°C Pleurotus ostreatus