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Literature summary for 1.10.3.2 extracted from

  • Khammuang, S.; Sarnthima, R.
    Laccase activity from fresh fruiting bodies of Ganoderma sp. MK05: Purification and remazol brilliant blue R decolorization (2009), J. Biol. Sci., 9, 83-87.
No PubMed abstract available

Organism

Organism UniProt Comment Textmining
Ganoderma sp.
-
-
-
Ganoderma sp. MK05
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 3fold by ammonium sulfate fractionation and anion exchange chromatography Ganoderma sp.
partial purification by ammonium sulfate precipitation and DEAE-cellulose column chromatography Ganoderma sp.

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
Ganoderma sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
partially purified enzyme Ganoderma sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Ganoderma sp. ?
-
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Ganoderma sp. MK05 ?
-
?
Remazol Brilliant Blue R + O2 the partial purified laccase shows a high efficient decolorization ability of Remazol Brilliant Blue R with 80.8% within 5 h at 30°C and pH 5.5 Ganoderma sp. ?
-
?
Remazol Brilliant Blue R + O2 the partial purified laccase shows a high efficient decolorization ability of Remazol Brilliant Blue R with 80.8% within 5 h at 30°C and pH 5.5 Ganoderma sp. MK05 ?
-
?

Synonyms

Synonyms Comment Organism
p-diphenol:O2 oxidoreductase
-
Ganoderma sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Ganoderma sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
assay at Ganoderma sp.
5.5
-
optimum pH for the decolorization ability of Remazol Brilliant Blue R Ganoderma sp.

pH Range

pH Minimum pH Maximum Comment Organism
3.7 8
-
Ganoderma sp.