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Literature summary for 1.10.3.2 extracted from
- Deletion and site-directed mutagenesis of laccase from Shigella dysenteriae results in enhanced enzymatic activity and thermostability (2009), Enzyme Microb. Technol., 44, 274-280.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as soluble intein fusion protein | Shigella dysenteriae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E106F | site-directed mutagenesis with additional deletion of residues L351-G378, the mutant WlacD shows 3.5fold increased activity and enhanced thermal stability compared to the wild-type enzyme | Shigella dysenteriae |
| E106F | site-directed mutagenesis, the mutant WlacS shows 2.2fold increased activity compared to the wild-type enzyme | Shigella dysenteriae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | completely inhibited by 200 mM | Shigella dysenteriae |  |
| NaN3 | completely inhibited by 50 mM | Shigella dysenteriae | |
| Pb2+ | inhibits 50% at 10 mM | Shigella dysenteriae | |
| SDS | completely inhibited by 32 mM | Shigella dysenteriae | |
| thiolglycolic acid | completely inhibited by 60 mM | Shigella dysenteriae | |
| Zn2+ | inhibits 40% at 10 mM | Shigella dysenteriae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.086 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, recombinant wild-type enzyme | Shigella dysenteriae | |
| 0.112 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, recombinant mutant WlacS | Shigella dysenteriae | |
| 0.129 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, recombinant mutant WlacD | Shigella dysenteriae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | stimulates the wild-type enzyme by 6% at 10 mM, 14.5% at 100 mM, required for activity | Shigella dysenteriae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 107000 | - |
recombinant mutant WlacD | Shigella dysenteriae |
| 110000 | - |
recombinant wild-type enzyme and mutant WlacS | Shigella dysenteriae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Shigella dysenteriae | - |
strain W202, a clinically isolated wild-type strain | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24.4 | - |
purified recombinant wild-type Wlac in presence of 100 mM Cu2+ | Shigella dysenteriae |
| 52.9 | - |
purified recombinant mutantWlacS in presence of 100 mM Cu2+ | Shigella dysenteriae |
| 85.1 | - |
purified recombinant mutantWlacD in presence of 100 mM Cu2+ | Shigella dysenteriae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Shigella dysenteriae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Wlac | - |
Shigella dysenteriae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Shigella dysenteriae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
25 | purified recombinant enzyme, stable | Shigella dysenteriae |
| 35 | - |
purified recombinant enzyme, 2 h, loss of 30% activity | Shigella dysenteriae |
| 45 | - |
purified recombinant enzyme, 1 h, loss of 30% activity | Shigella dysenteriae |
| 75 | - |
purified recombinant enzyme, 5 min, loss of 90% activity | Shigella dysenteriae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.2 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, recombinant wild-type enzyme | Shigella dysenteriae | |
| 36.5 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, recombinant mutant WlacS | Shigella dysenteriae | |
| 50.9 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, recombinant mutant WlacD | Shigella dysenteriae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
- |
Shigella dysenteriae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 0.5 | 8 | activity range | Shigella dysenteriae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
4-25°C, 24 h, loss of 40% activity | Shigella dysenteriae |
| 7 | - |
4-25°C, 24 h, stable | Shigella dysenteriae |
| 8 | - |
4°C, 24 h, loss of 25% activity, at 25°C oss of 55% activity | Shigella dysenteriae |
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