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Literature summary for 1.10.3.2 extracted from

  • Kaneko, S.; Cheng, M.; Murai, H.; Takenaka, S.; Murakami, S.; Aoki, K.
    Purification and characterization of an extracellular laccase from Phlebia radiata strain BP-11-2 that decolorizes fungal melanin (2009), Biosci. Biotechnol. Biochem., 73, 939-942.
    View publication on PubMed
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ complete inhibition at 1 mM Phlebia radiata
Fe3+ complete inhibition at 1 mM Phlebia radiata
Hg2+ strong inhibition at 1 mM Phlebia radiata

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Phlebia radiata
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+
-
 Phlebia radiata
additional information the enzyme activity is not affected by 7 mM Mn2+ and 1 mM H2O2, and by 1 mM of Ag+, Ba2+, Ca2+, Mg2+, Zn2+, Co2+, Ni2+ or Cu2+ or by sulfhydryl or chelating reagents Phlebia radiata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 gel filtration Phlebia radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
melanin + O2 Phlebia radiata the enzyme decolorizes fungal melanin from Alternaria alternata strain NBCR 31805 or black hyphae of Cladosporium sp. strain B-1, but not synthetic melanin, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Phlebia radiata
-
 isolated from forest soil
-
Phlebia radiata BP-11-2
-
 isolated from forest soil
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 250fold to homogeneity by anion exchange chromatographies and gel filtration Phlebia radiata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5
-
 purified native enzyme Phlebia radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Phlebia radiata ?
-
 ?
2,5-xylidine + O2
-
 Phlebia radiata ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Phlebia radiata ?
-
 ?
2-ethoxyphenol + O2
-
 Phlebia radiata ?
-
 ?
3,4-dimethoxybenzyl alcohol + O2
-
 Phlebia radiata ?
-
 ?
4-aminophenol + O2
-
 Phlebia radiata ?
-
 ?
4-methoxyphenol + O2
-
 Phlebia radiata ?
-
 ?
catechol + O2
-
 Phlebia radiata ?
-
 ?
catechol + O2
-
 Phlebia radiata BP-11-2 ?
-
 ?
guaiacol + O2
-
 Phlebia radiata ?
-
 ?
hydroquinone + O2
-
 Phlebia radiata ?
-
 ?
hydroquinone + O2
-
 Phlebia radiata BP-11-2 ?
-
 ?
L-tyrosine + O2
-
 Phlebia radiata ?
-
 ?
melanin + O2
-
 Phlebia radiata ?
-
 ?
melanin + O2 the enzyme decolorizes fungal melanin from Alternaria alternata strain NBCR 31805 or black hyphae of Cladosporium sp. strain B-1, but not synthetic melanin, overview Phlebia radiata ?
-
 ?
additional information the enzyme decolorizes fungal melanin and several other dye reagents, overview Phlebia radiata ?
-
 ?
additional information the enzyme decolorizes fungal melanin and several other dye reagents, overview Phlebia radiata BP-11-2 ?
-
 ?
p-cresol + O2
-
 Phlebia radiata ?
-
 ?
pyrogallol + O2
-
 Phlebia radiata ?
-
 ?
pyrogallol + O2
-
 Phlebia radiata BP-11-2 ?
-
 ?
resorcinol + O2
-
 Phlebia radiata ?
-
 ?
syringaldazine + O2
-
 Phlebia radiata ?
-
 ?
syringaldazine + O2
-
 Phlebia radiata BP-11-2 ?
-
 ?
vanillic acid + O2
-
 Phlebia radiata ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 x 61000 SDS-PAGE Phlebia radiata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Phlebia radiata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 purified enzyme, 10 min, 70% activity remaining Phlebia radiata
85
-
 purified enzyme, 10 min, inactivation Phlebia radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Phlebia radiata

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 7 purified enzyme, 4°C, 24 h, stable Phlebia radiata

Expression

Organism Comment Expression
Phlebia radiata the enzyme expression is induced by 2-ethoxyphenol, 2,5-xylidine, 4-methoxyphenol and p-cresol, and by melanin produced in Cladosporium sp. strain B-1 up