Application | Comment | Organism |
---|---|---|
additional information | the enzyme may be used in a variety of biotechnological applications, including textile dye bleaching, pulp bleaching, bioremediation, polymer synthesis and biosensors | Thermus thermophilus |
Cloned (Comment) | Organism |
---|---|
expression of a His-tagged two-type domain laccase, lacking the signal sequence domain, in Escherichia coli strain BL21(DE3) | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
two-domain-type by sitting-drop vapour-diffusion method, mixing of 0.001 ml of a protein solution, containing 8.5 mg/ml protein, with 0.001 ml of reservoir solution, containing 0.2 M potassium chloride, 0.05 M HEPES, pH 7.5, and 35% v/v pentaerythritol propoxylate, at 20°C, X-ray diffraction structure determination and analysis at 1.7 A resolution, single-wavelength anomalous diffraction technique using Cu atoms | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a two-type domain laccase from a three-type domain laccase by eliminating the signal sequence domain | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a multicopper protein | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermus thermophilus | the enzyme oxidizes a range of phenolic and nonphenolic compounds using molecular oxygen as an electron acceptor to produce water with a broad substrate specificity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
two-domain-type laccase from a metagenome | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged two-type domain laccase from Escherichia coli strain BL21(DE3) by heat treatment, centrifugation, nickel affinity chromatography, and ultrafiltration | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme oxidizes a range of phenolic and nonphenolic compounds using molecular oxygen as an electron acceptor to produce water with a broad substrate specificity | Thermus thermophilus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Benzenediol:oxygen oxidoreductase | - |
Thermus thermophilus |