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Literature summary for 1.10.3.2 extracted from

  • Park, K.M.; Park, S.S.
    Purification and characterization of laccase from basidiomycete Fomitella fraxinea (2008), J. Microbiol. Biotechnol., 18, 670-675.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, 43% inhibition Perenniporia fraxinea
Ba2+ 1 mM, 52% inhibition Perenniporia fraxinea
dithiothreitol 0.1 mM, 98% inhibition Perenniporia fraxinea
DMSO 10 mM, 34% inhibition Perenniporia fraxinea
EDTA 10 mM, 36% inhibition Perenniporia fraxinea
L-cysteine 0.1 mM, 95% inhibition Perenniporia fraxinea
Mn2+ 1 mM, 51% inhibition Perenniporia fraxinea
Ni2+ 1 mM, 57% inhibition Perenniporia fraxinea
SDS 10 mM, 21% inhibition Perenniporia fraxinea
Sodium azide 0.1 mM, 97% inhibition Perenniporia fraxinea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
-
Perenniporia fraxinea
0.426
-
2,6-dimethoxyphenol
-
Perenniporia fraxinea

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 1.14fold activation Perenniporia fraxinea
K+ 1 mM, 1.2fold activation Perenniporia fraxinea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
1 * 47000, SDS-PAGE Perenniporia fraxinea
47600
-
gel filtration Perenniporia fraxinea

Organism

Organism UniProt Comment Textmining
Perenniporia fraxinea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Perenniporia fraxinea

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Perenniporia fraxinea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
93.3
-
-
Perenniporia fraxinea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
-
Perenniporia fraxinea ? + H2O
-
?
2,6-dimethoxyphenol + O2
-
Perenniporia fraxinea ? + H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 47000, SDS-PAGE Perenniporia fraxinea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) Perenniporia fraxinea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 20°C: about 70% of maximal activity, 80°C: about 65% of maximal activity, oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) Perenniporia fraxinea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
pH 3, 1 h, about 15% loss of activity Perenniporia fraxinea
50
-
pH 3, 1 h, about 80% loss of activity Perenniporia fraxinea
60
-
pH 3, 1 h, complete loss of activity Perenniporia fraxinea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
103
-
2,6-dimethoxyphenol
-
Perenniporia fraxinea
208
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
-
Perenniporia fraxinea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) Perenniporia fraxinea

pH Range

pH Minimum pH Maximum Comment Organism
2.5 5 pH 2.5: about 85% of maximal activity, pH 5.0: about 50% of maximal activity, oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) Perenniporia fraxinea

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
25°C, 1 h, stable Perenniporia fraxinea
5
-
25°C, 1 h, about 15% loss of activity Perenniporia fraxinea
5.5
-
25°C, 1 h, about 55% loss of activity Perenniporia fraxinea
6
-
25°C, 1 h, about 80% loss of activity Perenniporia fraxinea

pI Value

Organism Comment pI Value Maximum pI Value
Perenniporia fraxinea isoelectric focusing, pH-range: 3-5
-
3.8