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Literature summary for 1.10.3.2 extracted from

  • Koschorreck, K.; Richter, S.M.; Ene, A.B.; Roduner, E.; Schmid, R.D.; Urlacher, V.B.
    Cloning and characterization of a new laccase from Bacillus licheniformis catalyzing dimerization of phenolic acids (2008), Appl. Microbiol. Biotechnol., 79, 217-224.
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0043
-
 syringaldazine pH 7.0 Bacillus licheniformis
0.0065
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) pH 4.0 Bacillus licheniformis
0.0567
-
 2,6-dimethoxyphenol pH 7.0 Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
-
 Bacillus licheniformis ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Bacillus licheniformis ?
-
 ?
caffeic acid + O2
-
 Bacillus licheniformis ?
-
 ?
ferulic acid + O2
-
 Bacillus licheniformis ?
-
 ?
additional information CotA fails to oxidize coumaric acid, cinnamic acid, and vanillic acid Bacillus licheniformis ?
-
 ?
sinapic acid + O2
-
 Bacillus licheniformis ?
-
 ?
syringaldazine + O2
-
 Bacillus licheniformis ?
-
 ?
syringic acid + O2
-
 Bacillus licheniformis 2,6-dimethoxy-1,4-benzoquinone + CO2 + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Bacillus licheniformis

Synonyms

Synonyms Comment Organism
CotA
-
 Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 1 h, 57% loss of activity Bacillus licheniformis
80
-
 1 h, 92% loss of activity Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 2,6-dimethoxyphenol pH 7.0 Bacillus licheniformis
83
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) pH 4.0 Bacillus licheniformis
100
-
 syringaldazine pH 7.0 Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
 oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) Bacillus licheniformis
7
-
 oxidation of syringaldazine or 2,6-dimethoxyphenol Bacillus licheniformis

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus licheniformis isoelectric focusing
-
 7