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Literature summary for 1.10.3.2 extracted from

  • Hilden, K.; Hakala, T.K.; Maijala, P.; Lundell, T.K.; Hatakka, A.
    Novel thermotolerant laccases produced by the white-rot fungus Physisporinus rivulosus (2007), Appl. Microbiol. Biotechnol., 77, 301-309.
    View publication on PubMed
Search for more literature for 1.10.3.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 syringaldazine pH 3.0, 25°C, laccase Lac-4.8 Ceriporiopsis rivulosa
0.0107
-
 2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate) pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
0.017
-
 2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate) pH 3.0, 25°C,laccase Lac-4.8 Ceriporiopsis rivulosa
0.027
-
 syringaldazine pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
0.084
-
 2,6-dimethoxyphenol pH 3.0, 25°C, laccase Lac-4.8 Ceriporiopsis rivulosa
0.088
-
 2,6-dimethoxyphenol pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
1.095
-
 guaiacol pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
1.406
-
 guaiacol pH 3.0, 25°C, laccase Lac-4.8 Ceriporiopsis rivulosa

Organism

Organism UniProt Comment Textmining
Ceriporiopsis rivulosa
-
-
-
Ceriporiopsis rivulosa T241i
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two laccases with distinct pI values, Lac-3.5 and Lac-4.8, are purified from peptone-spruce sawdust-charcoal cultures Ceriporiopsis rivulosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate) + O2
-
 Ceriporiopsis rivulosa ?
-
 ?
2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate) + O2
-
 Ceriporiopsis rivulosa T241i ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Ceriporiopsis rivulosa ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Ceriporiopsis rivulosa T241i ?
-
 ?
guaiacol + O2
-
 Ceriporiopsis rivulosa ?
-
 ?
guaiacol + O2
-
 Ceriporiopsis rivulosa T241i ?
-
 ?
syringaldazine + O2 i.e. 4-hydroxy-3,5-dimethoxybenzaldehyde azine Ceriporiopsis rivulosa ?
-
 ?
syringaldazine + O2 i.e. 4-hydroxy-3,5-dimethoxybenzaldehyde azine Ceriporiopsis rivulosa T241i ?
-
 ?

Synonyms

Synonyms Comment Organism
Lac-3.5
-
 Ceriporiopsis rivulosa
Lac-4.8
-
 Ceriporiopsis rivulosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 laccase Lac-4.8 shows thermal activation at 50°C and the enzyme activity after incubation is up to 120% of the original activity measured at 25°C Ceriporiopsis rivulosa
60
-
 Lac-3.5 and Lac-4.8 show thermal stability at up to 60°C Ceriporiopsis rivulosa
70
-
 half-life: 30 min, laccase Lac-4.8 Ceriporiopsis rivulosa
70
-
 half-life: 60 min, laccase Lac-3.5 Ceriporiopsis rivulosa
80
-
 20 min, 80% loss of activity Ceriporiopsis rivulosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
71
-
 guaiacol pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
82
-
 guaiacol pH 3.0, 25°C, laccase Lac-4.8 Ceriporiopsis rivulosa
124
-
 syringaldazine pH 3.0, 25°C, laccase Lac-4.8 Ceriporiopsis rivulosa
282
-
 syringaldazine pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
286
-
 2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate) pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
347
-
 2,6-dimethoxyphenol pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
409
-
 2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate) pH 3.0, 25°C, laccase Lac-4.8 Ceriporiopsis rivulosa
481
-
 2,6-dimethoxyphenol pH 3.0, 25°C, laccase Lac-4.8 Ceriporiopsis rivulosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 3.5 laccase Lac-3.5 Ceriporiopsis rivulosa
3 3.5 laccase Lac-4.8 Ceriporiopsis rivulosa

pI Value

Organism Comment pI Value Maximum pI Value
Ceriporiopsis rivulosa isoelectric focusing, laccase Lac-3.5
-
 3.5
Ceriporiopsis rivulosa isoelectric focusing, laccase Lac-4.8
-
 4.8