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Literature summary for 1.10.3.2 extracted from

  • Sahay, R.; Yadav, R.S.; Yadav, K.D.
    Purification and Characterization of Laccase Secreted by L. lividus (2008), Appl. Biochem. Biotechnol., 157, 311-320.
    View publication on PubMed
Search for more literature for 1.10.3.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 m-cresol
-
 Loweporus lividus MTCC-1178
0.21
-
 m-cresol in 50 mM sodium malonate buffer, pH 4.5 at 37°C Loweporus lividus
0.23
-
 catechol
-
 Loweporus lividus MTCC-1178
0.23
-
 catechol in 50 mM sodium malonate buffer, pH 4.5 at 37°C Loweporus lividus
0.32
-
 pyrogallol
-
 Loweporus lividus MTCC-1178
0.32
-
 pyrogallol in 50 mM sodium malonate buffer, pH 4.5 at 37°C Loweporus lividus
0.35
-
 syringaldazine
-
 Loweporus lividus MTCC-1178
0.35
-
 syringaldazine in 50 mM sodium malonate buffer, pH 4.5 at 37°C Loweporus lividus
0.48
-
 2,6-dimethoxyphenol
-
 Loweporus lividus MTCC-1178
0.48
-
 2,6-dimethoxyphenol in 50 mM sodium malonate buffer, pH 4.5 at 37°C Loweporus lividus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64800
-
-
 Loweporus lividus MTCC-1178
64800
-
 calculated from amino acid sequence Loweporus lividus
65000
-
 SDS-PAGE Loweporus lividus

Organism

Organism UniProt Comment Textmining
Loweporus lividus
-
-
-
Loweporus lividus MTCC-1178
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Loweporus lividus MTCC-1178
ammonium sulfate precipitation and DEAE column chromatography Loweporus lividus

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Loweporus lividus MTCC-1178
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.018
-
 crude enzyme, in 50 mM sodium malonate buffer, pH 4.5 at 37°C Loweporus lividus
1.41
-
-
 Loweporus lividus MTCC-1178
1.41
-
 after 67.6fold purification, in 50 mM sodium malonate buffer, pH 4.5 at 37°C Loweporus lividus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dimethoxyphenol + O2
-
 Loweporus lividus MTCC-1178 ? + H2O
-
 ?
2,6-dimethoxyphenol + O2
-
 Loweporus lividus 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
 ?
catechol + O2
-
 Loweporus lividus ?
-
 ?
catechol + O2
-
 Loweporus lividus MTCC-1178 ? + H2O
-
 ?
m-cresol + O2
-
 Loweporus lividus MTCC-1178 ? + H2O
-
 ?
m-cresol + O2
-
 Loweporus lividus ?
-
 ?
pyrogallol + O2
-
 Loweporus lividus ?
-
 ?
pyrogallol + O2
-
 Loweporus lividus MTCC-1178 ? + H2O
-
 ?
syringaldazine + O2
-
 Loweporus lividus MTCC-1178 ?
-
 ?
syringaldazine + O2 i.e. 2-hydroxy-3, 5-dimethoxybenaldehyde azine Loweporus lividus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 64800, SDS-PAGE Loweporus lividus MTCC-1178

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Loweporus lividus
60
-
 substrate: 2,6-dimethoxyphenol Loweporus lividus MTCC-1178

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 the enzyme retains more than 50% of its maximum activity at temperature above 40°C Loweporus lividus
40 70 40°C: about 35% of maximal activity, 70°C: about 35% of maximal activity, substrate: 2,6-dimethoxyphenol Loweporus lividus MTCC-1178

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 2 h, stable Loweporus lividus MTCC-1178
60
-
 the enzyme is stable at 60°C for 2 h Loweporus lividus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Loweporus lividus
5
-
 substrate: 2,6-dimethoxyphenol Loweporus lividus MTCC-1178

pH Range

pH Minimum pH Maximum Comment Organism
3 6 pH 3.0: about 45% of maximal activity, pH 6.0: about 35% of maximal activity, substrate: 2,6-dimethoxyphenol Loweporus lividus MTCC-1178
3.5 5.5 the enzyme retains more than 50% of its maximum activity in the pH range of 3.5-5.5 Loweporus lividus